{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:19:16Z","timestamp":1791425956010,"version":"4.3.3"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2012,4,10]],"date-time":"2012-04-10T00:00:00Z","timestamp":1334016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2012,5,2]],"date-time":"2012-05-02T00:00:00Z","timestamp":1335916800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2012,5,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The increasing antibiotic resistance of pathogenic\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    species and the absence of a pan-protective vaccine pose major health concerns. We recently identified, by subtractive reverse vaccinology, nine\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    antigens that protect mice from sepsis. In this study, we characterized one of them, ECOK1_0290, named FdeC (\n                    <jats:underline>f<\/jats:underline>\n                    actor a\n                    <jats:underline>d<\/jats:underline>\n                    herence\n                    <jats:italic>\n                      <jats:underline>E<\/jats:underline>\n                      .\u00a0\n                      <jats:underline>c<\/jats:underline>\n                      oli\n                    <\/jats:italic>\n                    ) for its ability to mediate\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    adhesion to mammalian cells and extracellular matrix. This adhesive propensity was consistent with the X-ray structure of one of the FdeC domains that shows a striking structural homology to\n                    <jats:named-content content-type=\"genus-species\">Yersinia pseudotuberculosis<\/jats:named-content>\n                    invasin and enteropathogenic\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    intimin. Confocal imaging analysis revealed that expression of FdeC on the bacterial surface is triggered by interaction of\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    with host cells. This phenotype was also observed in bladder tissue sections derived from mice infected with an extraintestinal strain. Indeed, we observed that FdeC contributes to colonization of the bladder and kidney, with the wild-type strain outcompeting the\n                    <jats:italic>fdeC<\/jats:italic>\n                    mutant in cochallenge experiments. Finally, intranasal mucosal immunization with recombinant FdeC significantly reduced kidney colonization in mice challenged transurethrally with uropathogenic\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    , supporting a role for FdeC in urinary tract infections.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    Pathogenic\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    strains are involved in a diverse spectrum of diseases, including intestinal and extraintestinal infections (urinary tract infections and sepsis). The absence of a broadly protective vaccine against all these\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    strains is a major problem for modern society due to high costs to health care systems. Here, we describe the structural and functional properties of a recently reported protective antigen, named FdeC, and elucidated its putative role during extraintestinal pathogenic\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    infection by using both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    infection models. The conservation of FdeC among strains of different\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    pathotypes highlights its potential as a component of a broadly protective vaccine against extraintestinal and intestinal\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    infections.\n                  <\/jats:p>","DOI":"10.1128\/mbio.00010-12","type":"journal-article","created":{"date-parts":[[2012,4,10]],"date-time":"2012-04-10T22:57:06Z","timestamp":1334098626000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":127,"title":["FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections"],"prefix":"10.1128","volume":"3","author":[{"given":"Barbara","family":"Nesta","sequence":"first","affiliation":[{"name":"Novartis Vaccines and Diagnostics Srl, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glen","family":"Spraggon","sequence":"additional","affiliation":[{"name":"The Genomics Institute of the Novartis Research Foundation, San Diego, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Alteri","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo","family":"Gomes Moriel","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics Srl, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Rosini","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics Srl, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Veggi","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics Srl, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Bertoldi","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics Srl, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Pastorello","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics Srl, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Ferlenghi","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics Srl, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rita","family":"Fontana","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics Srl, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gad","family":"Frankel","sequence":"additional","affiliation":[{"name":"Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry L. 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