{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:59:38Z","timestamp":1791331178030,"version":"4.3.1"},"reference-count":90,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Malin och Lennart Philipsons stiftelse","award":["LP Award"],"award-info":[{"award-number":["LP Award"]}]},{"DOI":"10.13039\/501100009252","name":"Science for Life Laboratory","doi-asserted-by":"publisher","award":["Post-doctoral grant"],"award-info":[{"award-number":["Post-doctoral grant"]}],"id":[{"id":"10.13039\/501100009252","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001729","name":"Stiftelsen f\u00f6r\u00a0Strategisk Forskning","doi-asserted-by":"publisher","award":["ICA16-0031"],"award-info":[{"award-number":["ICA16-0031"]}],"id":[{"id":"10.13039\/501100001729","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001729","name":"Stiftelsen f\u00f6r\u00a0Strategisk Forskning","doi-asserted-by":"publisher","award":["FFL18-0165"],"award-info":[{"award-number":["FFL18-0165"]}],"id":[{"id":"10.13039\/501100001729","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2018-02223"],"award-info":[{"award-number":["2018-02223"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2020-02342"],"award-info":[{"award-number":["2020-02342"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2022,2,22]]},"abstract":"<jats:p>Knowledge of dynamic niche-specific interactions between single microbes and host cells is essential to understand infectious disease progression. However, advances in this field have been hampered by the inherent conflict between the technical requirements for high-resolution live-cell imaging on the one hand and conditions that best mimic physiological infection niche parameters on the other.<\/jats:p>","DOI":"10.1128\/mbio.00022-22","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T05:00:47Z","timestamp":1643691647000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["High-Definition DIC Imaging Uncovers Transient Stages of Pathogen Infection Cycles on the Surface of Human Adult Stem Cell-Derived Intestinal Epithelium"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2865-4455","authenticated-orcid":true,"given":"Jorik M.","family":"van Rijn","sequence":"first","affiliation":[{"name":"Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8945-2665","authenticated-orcid":true,"given":"Jens","family":"Eriksson","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2507-3375","authenticated-orcid":true,"given":"Jana","family":"Gr\u00fcttner","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6243-2859","authenticated-orcid":true,"given":"Magnus","family":"Sundbom","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6979-9194","authenticated-orcid":true,"given":"Dominic-Luc","family":"Webb","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Gastroenterology and Hepatology Unit, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8428-0772","authenticated-orcid":true,"given":"Per M.","family":"Hellstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Gastroenterology and Hepatology Unit, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7392-1746","authenticated-orcid":true,"given":"Staffan G.","family":"Sv\u00e4rd","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8355-0803","authenticated-orcid":true,"given":"Mikael E.","family":"Sellin","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00348"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22126484"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002810"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13342"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.2.258-265.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1916676117"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09691"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.097386"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.07.050"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07935"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.12.042"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0259-3"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature19768"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1234852"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02684-20"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.11.041"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2013963118"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.98"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2013-306651"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5211"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2015.10.001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ab446e"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00733-18"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0333-1"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00740-18"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.03.021"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.05.007"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00003-21"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2021.08.007"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2016.07.019"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06948-x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13036-018-0111-x"},{"key":"e_1_3_2_34_2","first-page":"171","volume-title":"Methods in cell biology.","author":"van Dongen SFM","year":"2014","unstructured":"van Dongen SFM, Maiuri P, Piel M. 2014. Chapter 11. A reagent-based dynamic trigger for cell adhesion, shape change, or cocultures, p 171\u2013184. In Piel M, Th\u00e9ry M (ed), Methods in cell biology. Academic Press, New York, NY."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106904108"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.04.106"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.09.016"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2006989"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20558-6"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12739"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22156-6"},{"key":"e_1_3_2_42_2","first-page":"25","volume-title":"Advances in parasitology.","author":"Hagen KD","year":"2020","unstructured":"Hagen KD, McInally SG, Hilton ND, Dawson SC. 2020. Chapter two. Microtubule organelles in Giardia, p 25\u201396. In Ortega-Pierres MG (ed), Advances in parasitology. Academic Press, New York, NY."},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.88.012726"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00581-10"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00077-09"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00919.x"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15738-3"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008503"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12180"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00450.x"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1006098107"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.07.001"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00946-13"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002167"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000169"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2014.72"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.09.042"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2021.02.009"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.01.108"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00124.x"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01268.x"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.7.4138-4150.2004"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01218.x"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13107"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.108757"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008498"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00017-20"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-021-00381-y"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.03.016"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.13.1.11"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01458.x"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1002\/cm.10026"},{"key":"e_1_3_2_73_2","doi-asserted-by":"crossref","unstructured":"Chen J Lenaghan SC Zhang M. 2012. Analysis of dynamics and planar motion strategies of a swimming microorganism\u2014Giardia lamblia. IEEE Int Conf Robotics Automation p 4204\u20134209.","DOI":"10.1109\/ICRA.2012.6225014"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.44907"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2004.tb00165.x"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0278-0"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1655"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1038\/291238a0"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00830.x"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(83)90120-7"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00100-10"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00092-12"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.14440\/jbm.2014.36"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.8204-8208.2005"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_2_86_2","volume-title":"R: a language and environment for statistical computing.","author":"R Core Team.","year":"2021","unstructured":"R Core Team. 2021. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria."},{"key":"e_1_3_2_87_2","volume-title":"RStudio: integrated development environment for R.","author":"RStudio Team.","year":"2021","unstructured":"RStudio Team. 2021. RStudio: integrated development environment for R. R Studio, Boston, MA."},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.21105\/joss.02818"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.21105\/joss.01686"},{"key":"e_1_3_2_90_2","doi-asserted-by":"publisher","DOI":"10.1111\/eth.12739"},{"key":"e_1_3_2_91_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-050753-8.50064-4"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00022-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00022-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T23:01:13Z","timestamp":1646694073000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mbio.00022-22"}},"subtitle":[],"editor":[{"given":"Russell","family":"Vance","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,2,22]]},"references-count":90,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,2,22]]}},"alternative-id":["10.1128\/mbio.00022-22"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00022-22","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.08.24.457471","asserted-by":"object"}]},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,22]]},"assertion":[{"value":"2022-01-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00022-22"}}