{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T19:13:11Z","timestamp":1775157191439,"version":"3.50.1"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T00:00:00Z","timestamp":1552348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["272201400005C"],"award-info":[{"award-number":["272201400005C"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["5R21AI116285"],"award-info":[{"award-number":["5R21AI116285"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["PR17046"],"award-info":[{"award-number":["PR17046"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008091","name":"University of Rochester","doi-asserted-by":"publisher","award":["Drug Development and Technology Development Funds"],"award-info":[{"award-number":["Drug Development and Technology Development Funds"]}],"id":[{"id":"10.13039\/100008091","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,4,30]]},"abstract":"<jats:p>Influenza virus infections continue to cause substantial morbidity and mortality despite the availability of seasonal vaccines. The extensive genetic variability in seasonal and potentially pandemic influenza strains necessitates new vaccine strategies that can induce universal protection by focusing the immune response on generating protective antibodies against conserved targets such as regions within the influenza neuraminidase protein. We have demonstrated that seasonal immunization stimulates neuraminidase-specific antibodies in humans that are broad and potent in their protection from influenza B virus when tested in mice. These antibodies further persist in the bone marrow, where they are expressed by long-lived antibody-producing cells, referred to here as plasma cells. The significance in our research is the demonstration that seasonal influenza immunization can induce a subset of neuraminidase-specific B cells with broad protective potential, a process that if further studied and enhanced could aid in the development of a universal influenza vaccine.<\/jats:p>","DOI":"10.1128\/mbio.00066-19","type":"journal-article","created":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T10:43:59Z","timestamp":1552301039000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Broad and Protective Influenza B Virus Neuraminidase Antibodies in Humans after Vaccination and their Clonal Persistence as Plasma Cells"],"prefix":"10.1128","volume":"10","author":[{"given":"Michael S.","family":"Piepenbrink","sequence":"first","affiliation":[{"name":"Infectious Diseases Division, University of Rochester, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aitor","family":"Nogales","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Rochester, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhubanti","family":"Basu","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, University of Rochester, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher F.","family":"Fucile","sequence":"additional","affiliation":[{"name":"Informatics Institute, University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane L.","family":"Liesveld","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology\/James P. Wilmot Cancer Institute, University of Rochester, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael C.","family":"Keefer","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, University of Rochester, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander F.","family":"Rosenberg","sequence":"additional","affiliation":[{"name":"Informatics Institute, University of Alabama at Birmingham, Birmingham, Alabama, USA"},{"name":"Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Martinez-Sobrido","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Rochester, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James J.","family":"Kobie","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, University of Rochester, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis885"},{"key":"e_1_3_2_3_2","first-page":"1233","article-title":"Influenza-associated pediatric deaths\u2014United States, September 2010\u2013August 2011","volume":"60","author":"Centers for Disease Control and Prevention","year":"2011","unstructured":"Centers for Disease Control and Prevention. 2011. Influenza-associated pediatric deaths\u2014United States, September 2010\u2013August 2011. MMWR Morb Mortal Wkly Rep 60:1233\u20131238.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12522"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix393"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12432"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.08.023"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2017-2918"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2013.842466"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1707"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00190-4"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.144.3620.862"},{"key":"e_1_3_2_13_2","unstructured":"FDA. 2018. FDA approves new drug to treat influenza. https:\/\/www.fda.gov\/NewsEvents\/Newsroom\/PressAnnouncements\/ucm624226.htm. Accessed 5 January 2019."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-3-3-315"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10143"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0175655"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188267"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.10.041"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(57)90389-X"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1515\/bchm2.1955.301.1-2.235"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90003-T"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.8.6743-6751.1999"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-2659.2011.00304.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(74)90276-1"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.22.12665-12667.2004"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.21025"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2014.0061"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI84428"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/148.5.785"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1609316113"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.09.011"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-017-0011-8"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis935"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00417-16"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/129.4.411"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197206222862502"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv195"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.030"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.2.8.778-786.1968"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2012.06.003"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02556-14"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02434-12"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01584-17"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2015.11.014"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2014.12.006"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.05.049"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22307-8"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6964-7_14"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02473-14"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2016.10.001"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02281-16"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.06.090"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2016.02.021"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biologicals.2017.08.007"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01154-17"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01565-14"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01081-13"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01440-14"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.02.014"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2014.0064"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2007.09.017"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofu115"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(90)90255-T"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2014.09.003"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar3542"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.17226\/12910"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3175"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00339"},{"key":"e_1_3_2_69_2","volume-title":"PHYLIP (Phylogeny Inference Package) version 3.6.","author":"Felsenstein J","year":"2005","unstructured":"Felsenstein J. 2005. PHYLIP (Phylogeny Inference Package) version 3.6. Department of Genome Sciences, University of Washington, Seattle, WA."},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00066-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00066-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T02:29:29Z","timestamp":1646706569000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00066-19"}},"subtitle":[],"editor":[{"given":"Anne","family":"Moscona","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,4,30]]},"references-count":69,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,4,30]]}},"alternative-id":["10.1128\/mBio.00066-19"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00066-19","relation":{},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,30]]},"assertion":[{"value":"2019-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00066-19"}}