{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T04:05:27Z","timestamp":1776312327876,"version":"3.50.1"},"reference-count":111,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T00:00:00Z","timestamp":1619481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T00:00:00Z","timestamp":1619481600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Goverment of Spain","award":["BIO2016-75549-R"],"award-info":[{"award-number":["BIO2016-75549-R"]}]},{"name":"Goverment of Spain","award":["PID2019-107001RB-I00"],"award-info":[{"award-number":["PID2019-107001RB-I00"]}]},{"name":"Goverment of Spain","award":["202020E079"],"award-info":[{"award-number":["202020E079"]}]},{"name":"Goverment of Spain","award":["202020E043"],"award-info":[{"award-number":["202020E043"]}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["ZAPI_IMI_JU_115760"],"award-info":[{"award-number":["ZAPI_IMI_JU_115760"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["ISOLDA_848166"],"award-info":[{"award-number":["ISOLDA_848166"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["MANCO_101003651"],"award-info":[{"award-number":["MANCO_101003651"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["NIH_2P01AI060699"],"award-info":[{"award-number":["NIH_2P01AI060699"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2021,4,27]]},"abstract":"<jats:p>The emergence of the new highly pathogenic human coronavirus SARS-CoV-2 that has already infected more than 80 million persons, killing nearly two million of them, clearly indicates the need to design efficient and safe vaccines protecting from these coronaviruses. Modern vaccines can be derived from virus-host interaction research directed to the identification of signaling pathways essential for virus replication and for virus-induced pathogenesis, in order to learn how to attenuate these viruses and design vaccines.<\/jats:p>","DOI":"10.1128\/mbio.00103-21","type":"journal-article","created":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T13:56:03Z","timestamp":1614606963000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Genetically Engineered Live-Attenuated Middle East Respiratory Syndrome Coronavirus Viruses Confer Full Protection against Lethal Infection"],"prefix":"10.1128","volume":"12","author":[{"given":"Javier","family":"Guti\u00e9rrez-\u00c1lvarez","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.","family":"Honrubia","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal","family":"Fern\u00e1ndez-Delgado","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Casta\u00f1o-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Z\u00fa\u00f1iga","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Sola","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0854-0226","authenticated-orcid":true,"given":"Luis","family":"Enjuanes","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211721"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165978"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu359"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1306742"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)60454-8"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsr1408795"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2019.08.001"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2017.32.5.744"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2018.290"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30127-0"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2015.30.11.1701"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/v11010074"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001316"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00399-20"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.2003.012658"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001282"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.5582\/ddt.2020.01012"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25733"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2020.105949"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2020.105938"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2020.03.006"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2020.104787"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2020.105954"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21656"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1746200"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2006.01.017"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.60.080805.142157"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aivir.2016.08.008"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.03.003"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000188166.17324.60"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.5.2.245"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2147"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-017-0629-x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.04.016"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.10.012"},{"key":"e_1_3_3_37_2","unstructured":"Hewings-Martin Y. 2020. How do SARS and MERS compare with COVID-19? https:\/\/www.medicalnewstoday.com\/articles\/how-do-sars-and-mers-compare-with-covid-19."},{"key":"e_1_3_3_38_2","unstructured":"WHO. 2003. Summary of probable SARS cases with onset of illness from 1 November 2002 to 31 July 2003. World Health Organization Geneva Switzerland. https:\/\/www.who.int\/csr\/sars\/country\/table2004_04_21\/en\/."},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00473-12"},{"key":"e_1_3_3_40_2","unstructured":"Broadbent L. 2020. Coronaviruses \u2013 a brief history. https:\/\/theconversation.com\/coronaviruses-a-brief-history-135506."},{"key":"e_1_3_3_41_2","unstructured":"WHO. 2020. Middle East respiratory syndrome MERS situation update. http:\/\/www.emro.who.int\/health-topics\/mers-cov\/mers-outbreaks.html."},{"key":"e_1_3_3_42_2","unstructured":"CDC. 2020. Human coronavirus types. US Centers for Disease Control and Prevention Atlanta GA. https:\/\/www.cdc.gov\/coronavirus\/types.html."},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/v12020135"},{"key":"e_1_3_3_44_2","unstructured":"Kahn JS. 2020. New virus discovered by Chinese scientists investigating pneumonia outbreak. https:\/\/www.wsj.com\/articles\/new-virus-discovered-by-chinese-scientists-investigating-pneumonia-outbreak-11578485668."},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30120-1"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030685"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13077-2"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13412-5"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70204-4"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2014.09.003"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06048-11"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035421"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00983-14"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2016.1177688"},{"key":"e_1_3_3_55_2","first-page":"31","article-title":"SARS CoV subunit vaccine: antibody-mediated neutralisation and enhancement","volume":"18","author":"Jaume M","year":"2012","unstructured":"Jaume M, Yip MS, Kam YW, Cheung CY, Kien F, Roberts A, Li PH, Dutry I, Escriou N, Daeron M, Bruzzone R, Subbarao K, Peiris JS, Nal B, Altmeyer R. 2012. SARS CoV subunit vaccine: antibody-mediated neutralisation and enhancement. Hong Kong Med J 18(Suppl 2):31\u201336.","journal-title":"Hong Kong Med J"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aac7462"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.04.016"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01815-15"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.08.058"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00614-15"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01651-16"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2015.08.031"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep44875"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.02.063"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.3390\/v11030212"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01781"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1635"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aivir.2016.08.003"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2016.07.003"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004320"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03566-14"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02325-17"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.3390\/v11080682"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005215"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10-04-0338"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2011.03.029"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05070-11"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2000510"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens5010008"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv499"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-015-0720-y"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00650-13"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.04.029"},{"key":"e_1_3_3_84_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(00)80340-8"},{"key":"e_1_3_3_85_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(00)00114-3"},{"key":"e_1_3_3_86_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4291"},{"key":"e_1_3_3_87_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.151re12"},{"key":"e_1_3_3_88_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.23.4143"},{"key":"e_1_3_3_89_2","doi-asserted-by":"publisher","DOI":"10.1080\/1521654031000070636"},{"key":"e_1_3_3_90_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2009.02.012"},{"key":"e_1_3_3_91_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1296"},{"key":"e_1_3_3_92_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1300"},{"key":"e_1_3_3_93_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi201709w"},{"key":"e_1_3_3_94_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.09.007"},{"key":"e_1_3_3_95_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107309"},{"key":"e_1_3_3_96_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2014.05.023"},{"key":"e_1_3_3_97_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.642538"},{"key":"e_1_3_3_98_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-019-1182-0"},{"key":"e_1_3_3_99_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01467-06"},{"key":"e_1_3_3_100_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.10.7885-7894.1998"},{"key":"e_1_3_3_101_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00346-17"},{"key":"e_1_3_3_102_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01845-13"},{"key":"e_1_3_3_103_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03649-13"},{"key":"e_1_3_3_104_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005982"},{"key":"e_1_3_3_105_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00665-17"},{"key":"e_1_3_3_106_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006838"},{"key":"e_1_3_3_107_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00902-18"},{"key":"e_1_3_3_108_2","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1908.08004"},{"key":"e_1_3_3_109_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-019-9272-7"},{"key":"e_1_3_3_110_2","doi-asserted-by":"publisher","DOI":"10.2144\/97236bm04"},{"key":"e_1_3_3_111_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.18.8794"},{"key":"e_1_3_3_112_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00103-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00103-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T03:28:51Z","timestamp":1646710131000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00103-21"}},"subtitle":[],"editor":[{"given":"Mark R.","family":"Dennison","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,4,27]]},"references-count":111,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,4,27]]}},"alternative-id":["10.1128\/mBio.00103-21"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00103-21","relation":{},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,27]]},"assertion":[{"value":"2021-01-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00103-21"}}