{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T03:24:42Z","timestamp":1785727482227,"version":"3.56.0"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001502","name":"Department of Atomic Energy, Government of India","doi-asserted-by":"publisher","award":["DAE0195"],"award-info":[{"award-number":["DAE0195"]}],"id":[{"id":"10.13039\/501100001502","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology","doi-asserted-by":"publisher","award":["Salmonella Typhimurium (STM) resides in a membrane-bound compartment called Salmonella containing va"],"award-info":[{"award-number":["Salmonella Typhimurium (STM) resides in a membrane-bound compartment called Salmonella containing va"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"name":"TATA Innovation Fellowship"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,5,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n              Typhimurium (STM) resides in a membrane-bound compartment called the\n              <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n              -containing vacuole (SCV) in several infected cell types where bacterial and SCV division occur synchronously to maintain a single bacterium per vacuole. However, the mechanism behind this synchronous fission is not well understood. Fission of intracellular organelles is known to be regulated by the dynamic tubular endoplasmic reticulum (ER). In this study, we evaluated the role of ER in controlling SCV division. Interestingly,\n              <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n              -infected cells show activation of the unfolded protein response (UPR) and expansion of ER tubules. Altering the expression of ER morphology regulators, such as reticulon-4a (Rtn4a) and CLIMP63, significantly impacted bacterial proliferation, suggesting a potential role of tubular ER in facilitating SCV division. Live-cell imaging revealed the marking of tubular ER at the center of 78% of SCV division sites. This study also explored the role of SteA (a known\n              <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n              effector in modulating membrane dynamics) in coordinating the SCV division. SteA resides on the SCV membranes and helps form membrane contact between SCV and ER. The colocalization of ER with SCV enclosing STM\u0394\n              <jats:italic toggle=\"yes\">steA<\/jats:italic>\n              was significantly reduced, compared with SCV of STM WT or STM\u0394\n              <jats:italic toggle=\"yes\">steA:steA<\/jats:italic>\n              . STM\u0394\n              <jats:italic toggle=\"yes\">steA<\/jats:italic>\n              shows profound defects in SCV division, resulting in multiple bacteria in a single vacuole with proliferation defects.\n              <jats:italic toggle=\"yes\">In vivo<\/jats:italic>\n              , the STM\u0394\n              <jats:italic toggle=\"yes\">steA<\/jats:italic>\n              shows a defect in colonization in the spleen and liver and affects the initial survival rate of mice. Overall, this study suggests a coordinated role of bacterial effector SteA in promoting ER contact\/association with SCVs and regulating SCV division.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                This study highlights the essential role of the host endoplasmic reticulum in facilitating SCV division and maintaining a single bacterium per vacuole. The\n                <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                effector SteA helps maintain the single bacterium per vacuole state. In the absence of SteA,\n                <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                resides as multiple bacteria within a single large vacuole. The STM\u0394\n                <jats:italic toggle=\"yes\">steA<\/jats:italic>\n                shows reduced proliferation under\n                <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                conditions and exhibits colonization defects\n                <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                , highlighting the importance of this effector in\n                <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                pathogenesis. These findings suggest that targeting SteA could provide a novel therapeutic approach to inhibit\n                <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                pathogenicity.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/mbio.00114-25","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T13:01:38Z","timestamp":1745499698000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Endoplasmic reticulum facilitates the coordinated division of\n            <i>Salmonella<\/i>\n            -containing vacuoles"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2731-0810","authenticated-orcid":false,"given":"Umesh","family":"Chopra","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science","place":["Bangalore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyanka","family":"Bhansali","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science","place":["Bangalore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subba Rao","family":"Gangi Setty","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science","place":["Bangalore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7838-5145","authenticated-orcid":true,"given":"Dipshikha","family":"Chakravortty","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science","place":["Bangalore, India"]},{"name":"School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram","place":["Thiruvananthapuram, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2465-2467.2000"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1788"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2019.126311"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.11.005"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02230.x"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.8.2341-2344.2000"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(00)81442-7"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24983-z"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1286-4579(01)01490-3"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00830.x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.22.10544"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12475"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA119.001841"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00668-09"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.7.2232-2238.1991"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.15.4191"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.030301.x"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1207385"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1503191112"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature25486"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.6.1489"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.10.023"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201610057"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.8.2245"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1709060114"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086196"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.210799"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2221712120"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.18605"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800986200"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2010.01134.x"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.215855"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201607085"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2020.10.013"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9040705"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13050798"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.18.6755-6767.2000"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.07.018"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200406136"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan5835"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay7108"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.11.047"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.11553"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.08.030"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01385-13"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12558"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12382"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509157200"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.24.4637"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2016.10.008"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00068-09"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.10.5855-5870.2003"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002743"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.639019"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a010256"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1227037"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.11.011"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006700"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.100669"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2018-213532"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/9493019"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12641"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6260-6271.2005"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002092"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.04.021"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109434"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2021.08.012"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13176"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00114-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00114-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T13:02:37Z","timestamp":1747227757000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mbio.00114-25"}},"subtitle":[],"editor":[{"given":"Luis R.","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,5,14]]},"references-count":69,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5,14]]}},"alternative-id":["10.1128\/mbio.00114-25"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00114-25","relation":{},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,14]]},"assertion":[{"value":"2025-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00114-25"}}