{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T18:54:36Z","timestamp":1777748076333,"version":"3.51.4"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,5,29]],"date-time":"2012-05-29T00:00:00Z","timestamp":1338249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2012,7,2]],"date-time":"2012-07-02T00:00:00Z","timestamp":1341187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2012,7,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Susceptibility to mouse adenovirus type 1 (MAV-1) is mouse strain dependent; susceptible mice die from hemorrhagic encephalomyelitis. The MAV-1 susceptibility quantitative trait locus<jats:italic>Msq1<\/jats:italic>accounts for ~40% of the phenotypic (brain viral load) variance that occurs between resistant BALB\/c and susceptible SJL mice after MAV-1 infection. Using an interval-specific congenic mouse strain (C.SJL-Msq1<jats:sup>SJL<\/jats:sup>), in which the SJL-derived allele<jats:italic>Msq1<jats:sup>SJL<\/jats:sup><\/jats:italic>is present in a BALB\/c background, we demonstrate that<jats:italic>Msq1<jats:sup>SJL<\/jats:sup><\/jats:italic>controls the development of high brain viral titers in response to MAV-1 infection, yet does not account for the total extent of brain pathology or mortality in SJL mice. C.SJL-Msq1<jats:sup>SJL<\/jats:sup>mice had disruption of the blood-brain barrier and increased brain water content after MAV-1 infection, but these effects occurred later and were not as severe, respectively, as those noted in infected SJL mice. As expected, BALB\/c mice showed minimal pathology in these assays. Infection of SJL- and C.SJL-Msq1<jats:sup>SJL<\/jats:sup>-derived primary mouse brain endothelial cells resulted in loss of barrier properties, whereas BALB\/c-derived cells retained their barrier properties despite being equally capable of supporting MAV-1 infection. Finally, we provide evidence that organ pathology and inflammatory cell recruitment to the brain following MAV-1 infection were both influenced by<jats:italic>Msq1<\/jats:italic>. These results validate<jats:italic>Msq1<\/jats:italic>as an important host factor in MAV-1 infection and refine the major role of the locus in development of MAV-1 encephalitis. They further suggest that additional host factors or gene interactions are involved in the mechanism of pathogenesis in MAV-1-infected SJL mice.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>A successful viral infection requires both host and viral factors; identification of host components involved in viral replication and pathogenesis is important for development of therapeutic interventions. A genetic locus (<jats:italic>Msq1<\/jats:italic>) controlling mouse adenovirus type 1 (MAV-1) brain infection was previously identified. Genes in<jats:italic>Msq1<\/jats:italic>belong to the same family of genes associated with susceptibility to other encephalitic viruses, HIV-1 and West Nile virus. We constructed an interval-specific congenic mouse strain to examine the contribution of<jats:italic>Msq1<\/jats:italic>to MAV-1 susceptibility and brain morbidity. We compared infected resistant, susceptible, and congenic mice regarding known MAV-1 disease manifestations in the brain (survival, viral loads, blood-brain barrier disruption, edema, mouse brain endothelial cell barrier properties, pathology, and inflammatory cell recruitment) to determine the extent to which<jats:italic>Msq1<\/jats:italic>influences MAV-1 infection outcome. Our results showed that<jats:italic>Msq1<\/jats:italic>is a critical host genetic factor that controls many aspects of MAV-1 infection.<\/jats:p>","DOI":"10.1128\/mbio.00131-12","type":"journal-article","created":{"date-parts":[[2012,5,30]],"date-time":"2012-05-30T05:50:50Z","timestamp":1338357050000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Contribution of a Single Host Genetic Locus to Mouse Adenovirus Type 1 Infection and Encephalitis"],"prefix":"10.1128","volume":"3","author":[{"given":"Tien-Huei","family":"Hsu","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene W.","family":"Althaus","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oded","family":"Foreman","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, Sacramento, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine R.","family":"Spindler","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Fields virology","author":"Fields BN","year":"2007","unstructured":"FieldsBN KnipeDM HowleyPM . 2007. Fields virology, 5th ed. Wolters Kluwer Health\/Lippincott Williams and Wilkins, Philadelphia, PA.","edition":"5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-72-4-859"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65511-3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20080421"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00954-09"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/13550280902998413"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.10.036"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2009.07.030"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.2.4"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.3929"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00691095"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/64.6.498"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82093-0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.5.1.49"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00052-07"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-0981-7_13"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(97)00015-6"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.12.7674-7681.1995"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.12.8084-8088.1995"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22131"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-S1-S9"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9180"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.24.12039-12046.2001"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.17.11517-11522.2005"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903363"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1992.72.3.865"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1943.138.5.708"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1053\/snep.2001.21665"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.2.1219-1223.1998"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-0987(00)00123-8"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00727-09"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83345-0"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.033845"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(90)90003-V"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.13.6898"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.1.115"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"4233","DOI":"10.4049\/jimmunol.154.9.4233","article-title":"Regulated expression of Ly-6A.2 is important for T cell development","volume":"154","author":"Bamezai A","year":"1995","unstructured":"BamezaiA . 1995. Regulated expression of Ly-6A.2 is important for T cell development. J. Immunol. 154:4233\u20134239.","journal-title":"J. Immunol"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.6.3874"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10301"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.05.025"},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4049\/jimmunol.156.1.1","article-title":"Matrix metalloproteinases in immunity","volume":"156","author":"Goetzl EJ","year":"1996","unstructured":"GoetzlEJ BandaMJ LeppertD . 1996. Matrix metalloproteinases in immunity. J. Immunol. 156:1\u20134.","journal-title":"J. Immunol"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.11.5584-5590.2004"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.18.10060-10070.2003"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/51.3.977"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcs013"},{"key":"e_1_3_2_47_2","first-page":"192","article-title":"Pathogenesis of HIV encephalitis","volume":"41","author":"Wiley CA","year":"1991","unstructured":"WileyCA . 1991. Pathogenesis of HIV encephalitis. Acta Pathol. Jpn. 41:192\u2013196.","journal-title":"Acta Pathol. Jpn"},{"key":"e_1_3_2_48_2","first-page":"329","article-title":"The invasion of the brain by yellow fever virus present in the blood of mice","volume":"38","author":"Mims CA","year":"1957","unstructured":"MimsCA . 1957. The invasion of the brain by yellow fever virus present in the blood of mice. Br. J. Exp. Pathol. 38:329\u2013338.","journal-title":"Br. J. Exp. Pathol"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1159\/000075767"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.1.533-539.2001"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.07.013"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07207"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09907"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060032"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152725"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90030-F"},{"key":"e_1_3_2_57_2","first-page":"215","article-title":"Capture ELISA quantitation of mouse adenovirus type 1 in infected organs","volume":"130","author":"Welton AR","year":"2007","unstructured":"WeltonAR SpindlerKR . 2007. Capture ELISA quantitation of mouse adenovirus type 1 in infected organs. Methods Mol. Med. 130:215\u2013221.","journal-title":"Methods Mol. Med"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.12.7666"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600055"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00131-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00131-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T00:34:52Z","timestamp":1687480492000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00131-12"}},"subtitle":[],"editor":[{"given":"Terence S.","family":"Dermody","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2012,7,2]]},"references-count":59,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,7,2]]}},"alternative-id":["10.1128\/mBio.00131-12"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00131-12","relation":{},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,2]]},"assertion":[{"value":"2012-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-05-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-05-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00131-12"}}