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Single-virus tracking is a versatile technique to study these events. To perform this technique, viruses must be fluorescently labeled to be visible to fluorescence microscopes. The quantum dot is a kind of fluorescent tag that has many unique optical properties. It has been widely used to label proteins in single-molecule-tracking studies but rarely used to study virus infection, mainly due to the lack of an accepted labeling method. In this study, we developed a lipid-specific method to readily, mildly, specifically, and efficiently label enveloped viruses with quantum dots by recognizing viral envelope lipids with lipid-biotin conjugates and recognizing these lipid-biotin conjugates with streptavidin-quantum dot conjugates. It is not only applicable to normal viruses, but also competent to label the key protein-mutated viruses and the inactivated highly virulent viruses, providing a powerful tool for single-virus tracking.<\/jats:p>","DOI":"10.1128\/mbio.00135-20","type":"journal-article","created":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T09:18:21Z","timestamp":1589793501000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Lipid-Specific Labeling of Enveloped Viruses with Quantum Dots for Single-Virus Tracking"],"prefix":"10.1128","volume":"11","author":[{"given":"Li-Juan","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, Wuhan Institute of Biotechnology, Wuhan University, Wuhan, People\u2019s Republic of 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