{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T22:55:56Z","timestamp":1793141756741,"version":"4.5.0"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T00:00:00Z","timestamp":1558396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T00:00:00Z","timestamp":1561420800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Max Planck Society"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,6,25]]},"abstract":"<jats:p>\n            Tuberculosis is caused by\n            <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n            , and the increasing emergence of multidrug-resistant strains renders current treatment options ineffective. Although new antimycobacterial drugs are urgently required, their successful development often relies on complete understanding of the metabolic pathways\u2014e.g., cholesterol assimilation\u2014that are critical for persistence and for pathogenesis of\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            . In this regard, mycofactocin (MFT) function appears to be important because its biosynthesis genes are predicted to be essential for\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            <jats:italic>in vitro<\/jats:italic>\n            growth in cholesterol. In determining the metabolic basis of this genetic requirement, our results unexpectedly revealed the essential function of MFT in ethanol metabolism. The metabolic dysfunction thereof was found to affect the mycobacterial growth in cholesterol which is solubilized by ethanol. This knowledge is fundamental in recognizing the bona fide function of MFT, which likely resembles the pyrroloquinoline quinone-dependent ethanol oxidation in acetic acid bacteria exploited for industrial production of vinegar.\n          <\/jats:p>","DOI":"10.1128\/mbio.00190-19","type":"journal-article","created":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T14:09:45Z","timestamp":1558361385000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Mycofactocin Is Associated with Ethanol Metabolism in Mycobacteria"],"prefix":"10.1128","volume":"10","author":[{"given":"Gopinath","family":"Krishnamoorthy","sequence":"first","affiliation":[{"name":"Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peggy","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Lozza","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Hahnke","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Joachim","family":"Mollenkopf","sequence":"additional","affiliation":[{"name":"Core Facility Microarray\/Genomics, Max Planck Institute for Infection Biology, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan H. 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