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In this study, we identified\n                    <jats:italic>MGE1<\/jats:italic>\n                    as a multicopy suppressor of susceptibility to the antifungal fluconazole in the model yeast\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    . We demonstrate that this phenomenon is not exclusively dependent on the integrity of the mitochondrial DNA or on the presence of the drug efflux pump Pdr5. Instead, we show that the increased dosage of Mge1 plays a protective role by retaining increased amounts of ergosterol upon fluconazole treatment. Iron metabolism and, more particularly, Fe-S cluster formation are involved in regulating this process, since the responsible Hsp70 chaperone, Ssq1, is required. Additionally, we show the necessity but, by itself, insufficiency of activating the iron regulon in establishing the Mge1-related effect on drug susceptibility. Finally, we confirm a similar role for Mge1 in fluconazole susceptibility in the pathogenic fungi\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    .\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    Although they are mostly neglected compared to bacterial infections, fungal infections pose a serious threat to the human population. While some of them remain relatively harmless, infections that reach the bloodstream often become lethal. Only a few therapies are available, and resistance of the pathogen to these drugs is a frequently encountered problem. It is thus essential that more research is performed on how these pathogens cope with the treatment and cause recurrent infections. Baker\u2019s yeast is often used as a model to study pathogenic fungi. We show here, by using this model, that iron metabolism and the formation of the important iron-sulfur clusters are involved in regulating susceptibility to fluconazole, the most commonly used antifungal drug. We show that the same process likely also occurs in two of the most regularly isolated pathogenic fungi,\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/mbio.00201-17","type":"journal-article","created":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T20:10:19Z","timestamp":1500408619000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Mitochondrial Cochaperone Mge1 Is Involved in Regulating Susceptibility to Fluconazole in\n                    <i>Saccharomyces cerevisiae<\/i>\n                    and\n                    <i>Candida<\/i>\n                    Species"],"prefix":"10.1128","volume":"8","author":[{"given":"Liesbeth","family":"Demuyser","sequence":"first","affiliation":[{"name":"VIB-KU Leuven Center for 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