{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:32:27Z","timestamp":1791995547444,"version":"4.4.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2011,10,18]],"date-time":"2011-10-18T00:00:00Z","timestamp":1318896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Understanding the molecular pathogenesis of\n            <jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>\n            , the causative agent of human Q fever, has historically been hindered by the technical difficulties of genetically manipulating obligate intracellular bacteria. The recent development of culture conditions suitable for axenic propagation of\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            has paved the way for the application of a range of genetic techniques to address key questions within the field. Recent studies using mutational analysis have revealed that the\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            Dot\/Icm type 4 secretion system (T4SS) is an important virulence determinant that is essential for renovation of a lysosome into a mature\n            <jats:italic>Coxiella<\/jats:italic>\n            -containing vacuole (CCV) permissive of intracellular replication. Interestingly, a mutant of\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            deficient in Dot\/Icm function was found to be capable of replicating within the parasitophorous vacuole created by\n            <jats:named-content content-type=\"genus-species\">Leishmania amazonensis<\/jats:named-content>\n            , which indicates that\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            replication is not dependent on the cohort of Dot\/Icm effector proteins\n            <jats:italic>per se<\/jats:italic>\n            but rather that the collective actions of effectors are required to create the specialized niche supportive of replication. Thus, a role for the Dot\/Icm T4SS during the intracellular life cycle of\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            has been more clearly defined by these studies, which demonstrate that advances in genetic analysis should allow future studies to focus on the intricacies of Dot\/Icm effector functions that facilitate development of the unique CCV.\n          <\/jats:p>","DOI":"10.1128\/mbio.00226-11","type":"journal-article","created":{"date-parts":[[2011,10,19]],"date-time":"2011-10-19T03:46:03Z","timestamp":1318995963000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["The Coxiella burnetii Dot\/Icm System Creates a Comfortable Home through Lysosomal Renovation"],"prefix":"10.1128","volume":"2","author":[{"given":"Hayley J.","family":"Newton","sequence":"first","affiliation":[{"name":"Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig R.","family":"Roy","sequence":"additional","affiliation":[{"name":"Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00136"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03626.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.7.3714-3723.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100109-104034"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00175-11"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002056"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010485107"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1158160"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01359-10"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01656-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01911-07"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812074106"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01580-08"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00097"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00841.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/15687"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01141-08"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1447"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1111678108"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000501"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406239101"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00301-09"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00838.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000220"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01145.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.9.3502-3506.1995"},{"key":"e_1_3_2_28_2","article-title":"Metabolic pathways required for the intracellular survival of Leishmania","author":"McConville MJ","unstructured":"McConvilleMJ NadererT . 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