{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T01:15:34Z","timestamp":1760577334461,"version":"3.41.2"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,5,7]],"date-time":"2013-05-07T00:00:00Z","timestamp":1367884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Immunity to\n            <jats:named-content content-type=\"genus-species\">Campylobacter jejuni<\/jats:named-content>\n            , a major diarrheal pathogen, is largely Penner serotype specific. For broad protection, a vaccine should be based on a common antigen(s) present in all strains. In our previous study (M. J. Albert, S. Haridas, D. Steer, G. S. Dhaunsi, A. I. Smith, and B. Adler, Infect. Immun. 75:3070\u20133073, 2007), we demonstrated that antibody to cholera toxin (CT) cross-reacted with the major outer membrane proteins (MOMPs) of all\n            <jats:named-content content-type=\"genus-species\">Campylobacter jejuni<\/jats:named-content>\n            strains tested. In the current study, we investigated whether immunization with CT protects against intestinal colonization by\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            in an adult mouse model and whether the nontoxic subunit of CT (CT-B) is the portion mediating cross-reaction. Mice were orally immunized with CT and later challenged with\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            strains (48, 75, and 111) of different serotypes. Control animals were immunized with phosphate-buffered saline. Fecal shedding of challenge organisms was studied daily for 9 days. Serum and fecal antibody responses were studied by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. The cross-reactivity of rabbit CT-B antibody to MOMP was studied by immunoblotting. The reactivity of 21 overlapping 30-mer oligopeptides (based on MOMP\u2019s sequence) against rabbit CT antibody was tested by ELISA. Test animals produced antibodies to CT and MMP in serum and feces and showed resistance to colonization, the vaccine efficacies being 49% (for strain 48), 37% (for strain 75), and 34% (for strain 111) (\n            <jats:italic>P<\/jats:italic>\n            , \u22640.05 to \u22640.001). One peptide corresponding to a variable region of MOMP showed significant reactivity. CT-B antibody cross-reacted with MOMP. Since CT-B is a component of oral cholera vaccines, it might be possible to control\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            diarrhea with these vaccines.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            <jats:named-content content-type=\"genus-species\">Campylobacter jejuni<\/jats:named-content>\n            is a major cause of diarrhea worldwide. Patients who recover from\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            diarrhea develop immunity to the infecting serotype and remain susceptible to infection with other serotypes. A vaccine based on a common protective antigen(s) present in all\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            serotypes is expected to provide broad protection. In our previous study, we showed that antibody to cholera toxin (CT) reacted with the major outer membrane proteins (MOMPs) from different strains of\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            . We assumed that the B subunit of the toxin (CT-B), which is nontoxic and a component of licensed oral cholera vaccines, might be the component that cross-reacts with MOMP. In the current study, we showed that orally immunizing mice with CT protected them against colonization upon challenge with different serotypes of\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            . We also showed that CT-B is the component mediating cross-reaction. Therefore, it might be possible to use cholera vaccines to prevent\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            diarrhea. This could result in significant savings in vaccine development and treatment of the disease.\n          <\/jats:p>","DOI":"10.1128\/mbio.00246-13","type":"journal-article","created":{"date-parts":[[2013,5,8]],"date-time":"2013-05-08T04:09:39Z","timestamp":1367986179000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Oral Immunization with Cholera Toxin Provides Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model"],"prefix":"10.1128","volume":"4","author":[{"given":"M. John","family":"Albert","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abu Salim","family":"Mustafa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anjum","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shilpa","family":"Haridas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"doi-asserted-by":"crossref","unstructured":"NewellDG\nKoopmansM\nVerhoefL\nDuizerE\nAidara-KaneA\nSprongH\nOpsteeghM\nLangelaarM\nThrefallJ\nScheutzF\nvan der GiessenJ\nKruseH\n. 2010. Food-borne diseases\u2014the challenge of 20 years ago still persists while new ones continue to emerge. Int. J. Food Microbiol. 139(Suppl 1):S3\u2013S15. http:\/\/dx.doi.org\/10.1016\/j.ijfoodmicro.2010.01.021.","key":"e_1_3_2_2_2","DOI":"10.1016\/j.ijfoodmicro.2010.01.021"},{"key":"e_1_3_2_3_2","first-page":"121","volume-title":"Campylobacter","author":"Friedman CR","year":"2000","unstructured":"FriedmanCR NeimannJ WegenerHC TauxeRV . 2000. Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations, p 121\u2013138. In NachamkinI BlaserMJ , Campylobacter, 2nd ed. ASM Press, Washington, DC.","edition":"2"},{"key":"e_1_3_2_4_2","first-page":"139","volume-title":"Campylobacter","author":"Oberhelman RA","year":"2000","unstructured":"OberhelmanRA TaylorDN . 2000. Campylobacter infections in developing countries, p 139\u2013154. In NachamkinI BlaserMJ , Campylobacter, 2nd ed. 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