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Compounding evidence indicates pregnant individuals are particularly vulnerable to RVFV, as\n                      <jats:italic toggle=\"yes\">in utero<\/jats:italic>\n                      transmission and increased rates of miscarriage have been reported in people infected during pregnancy. Further, human placenta explants are permissive to RVFV infection. Viable vaccine candidates intended for veterinary or human use must protect vulnerable populations, including pregnant individuals and fetuses. Using a pregnant rat model, we show that maternal vaccination with a live-attenuated RVFV lacking the non-structural proteins NSs and NSm (RVFV-delNSs\/NSm) is immunogenic, safe, and protective. Dams vaccinated either prior to or during pregnancy were protected from virulent challenge during pregnancy, and we found no evidence of infectious virus in the placentas and fetuses of challenged animals. These studies offer important pre-clinical data in a tractable pregnancy model and serve as a blueprint for evaluating future vaccine approaches designed to protect pregnant individuals and their fetuses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Rift Valley fever virus (RVFV) poses a formidable threat to pregnant animals and potentially to pregnant individuals and their developing fetuses. Despite this risk, pregnant individuals are rarely included in vaccine trials, leaving this potentially vulnerable population unprotected. Using a live-attenuated RVFV vaccine, we demonstrate that maternal vaccination is safe and protects both pregnant rats and their fetuses from congenital Rift Valley fever. Because live-attenuated vaccines are often contraindicated during pregnancy due to theoretical safety concerns, we further show that vaccination prior to pregnancy provides equivalent protection throughout future pregnancy. Taken together, these findings provide a blueprint for the preclinical evaluation of RVFV vaccines during pregnancy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.00253-26","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T12:00:52Z","timestamp":1782129652000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Maternal vaccination protects dams and prevents\n                    <i>in utero<\/i>\n                    transmission of Rift Valley fever virus in rats"],"prefix":"10.1128","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4355-855X","authenticated-orcid":false,"given":"Austin T.","family":"Hertel","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vaccine Research, University of Pittsburgh","place":["Pittsburgh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9211-7908","authenticated-orcid":true,"given":"Cynthia M.","family":"McMillen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vaccine Research, University of Pittsburgh","place":["Pittsburgh, USA"]},{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Disease and Microbiology, School of Public Health, University of Pittsburgh","place":["Pittsburgh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan M.","family":"Hoehl","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vaccine Research, University of Pittsburgh","place":["Pittsburgh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary D.","family":"Frey","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vaccine Research, University of Pittsburgh","place":["Pittsburgh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6764-7536","authenticated-orcid":true,"given":"Anita K.","family":"McElroy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vaccine Research, University of Pittsburgh","place":["Pittsburgh, USA"]},{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Pediatric Infectious Disease, Department of Pediatrics, School of Medicine, University of Pittsburgh","place":["Pittsburgh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0857-2973","authenticated-orcid":true,"given":"Amy L.","family":"Hartman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vaccine Research, University of Pittsburgh","place":["Pittsburgh, USA"]},{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Disease and Microbiology, School of Public Health, University of Pittsburgh","place":["Pittsburgh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","unstructured":"World Health Organization. 2024. 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