{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T14:05:36Z","timestamp":1793109936801,"version":"4.5.0"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01-AI021423"],"award-info":[{"award-number":["R01-AI021423"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01-AI129529"],"award-info":[{"award-number":["R01-AI129529"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["T32-AI732832"],"award-info":[{"award-number":["T32-AI732832"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE-114747"],"award-info":[{"award-number":["DGE-114747"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["F32-HL154711"],"award-info":[{"award-number":["F32-HL154711"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01-DK12140903"],"award-info":[{"award-number":["R01-DK12140903"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006919","name":"Massachusetts Institute of Technology","doi-asserted-by":"publisher","award":["Lester Wolfe Fellowship"],"award-info":[{"award-number":["Lester Wolfe Fellowship"]}],"id":[{"id":"10.13039\/100006919","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006919","name":"Massachusetts Institute of Technology","doi-asserted-by":"publisher","award":["Dow Graduate Research Fellowship"],"award-info":[{"award-number":["Dow Graduate Research Fellowship"]}],"id":[{"id":"10.13039\/100006919","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE-1656518"],"award-info":[{"award-number":["DGE-1656518"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2021,12,21]]},"abstract":"<jats:p>\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    is an intracellular pathogen which resides and replicates inside a membrane-bound vacuole in infected cells. This vacuole is modified by both parasite and host proteins which participate in a variety of host-parasite interactions at this interface, including nutrient exchange, effector transport, and immune modulation.\n                  <\/jats:p>","DOI":"10.1128\/mbio.00260-21","type":"journal-article","created":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T04:30:29Z","timestamp":1636432229000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Proximity-Labeling Reveals Novel Host and Parasite Proteins at the\n                    <i>Toxoplasma<\/i>\n                    Parasitophorous Vacuole Membrane"],"prefix":"10.1128","volume":"12","author":[{"given":"Alicja M.","family":"Cygan","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford School of Medicine, Stanford California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre M.","family":"Jean Beltran","sequence":"additional","affiliation":[{"name":"Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma G.","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford School of Medicine, Stanford California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tess C.","family":"Branon","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Y.","family":"Ting","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven A.","family":"Carr","sequence":"additional","affiliation":[{"name":"Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9719-745X","authenticated-orcid":true,"given":"John C.","family":"Boothroyd","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford School of Medicine, Stanford California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1079\/ahr2005100"},{"key":"e_1_3_2_3_2","first-page":"53","article-title":"Behaviour of microtubules in cells infected with Toxoplasma gondii","volume":"25","author":"Melo EJ","year":"2001","unstructured":"Melo EJ, Carvalho TM, De Souza W. 2001. Behaviour of microtubules in cells infected with Toxoplasma gondii. Biocell 25:53\u201359.","journal-title":"Biocell"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01061.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.08.014"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.136.5.1173"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.17.2117"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.3.908"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.149.1.167"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1998.00553.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00005-17"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00402-20"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.04.002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00045-16"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2019.07.002"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2017.02.007"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00808-16"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00626"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4201"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201112098"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2009.00958.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.4.1669"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.136.3.531"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051629998"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0504-8"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.09.011"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.12.3132"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001845"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90015-p"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00767.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0166-6851(99)00092-4"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0166-6851(99)00160-7"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02699-19"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm256"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406459111"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10809"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0177-4"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-017-0018-1"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17091401"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201745453"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.019"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11855-w"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01339-13"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.09.008"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18991-8"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2016.08.016"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2012.02.044"},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","unstructured":"Rivera-Cuevas Y Mayoral J Di Cristina M Lawrence A-LE Olafsson EB Thornhill D Waldman BS Ono A Sexton J Lourido S Weiss LM Carruthers VB. 2021. Toxoplasma gondii subverts the host ESCRT machinery for parasite uptake of host cytosolic proteins. bioRxiv 2021.07.21.453261.","DOI":"10.1101\/2021.07.21.453261"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b04396"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00276-19"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000309"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504158200"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00357-08"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006828"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1133690"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1061888"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01667-07"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1111\/1467-9868.00346"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz369"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00702"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_3_2_63_2","doi-asserted-by":"crossref","unstructured":"Saffer LD Mercereau-Puijalon O Dubremetz JF Schwartzman JD. 1992. Localization of a Toxoplasma gondii rhoptry protein by immunoelectron microscopy during and after host cell penetration. J Protozool 39(4):526\u201330. doi:10.1111\/j.1550-7408.1992.tb04844.x.","DOI":"10.1111\/j.1550-7408.1992.tb04844.x"},{"key":"e_1_3_2_64_2","doi-asserted-by":"crossref","unstructured":"Lecordier L Mercier C Sibley LD Cesbron-Delauw MF. 1999. Transmembrane insertion of the Toxoplasma gondii GRA5 protein occurs after soluble secretion into the host cell. Mol Biol Cell 10(4):1277\u20131287. doi:10.1091\/mbc.10.4.1277.","DOI":"10.1091\/mbc.10.4.1277"},{"key":"e_1_3_2_65_2","doi-asserted-by":"crossref","unstructured":"Ferguson DJ Parmley SF. 2002. Toxoplasma gondii MAG1 protein expression. Trends Parasitol 18(11):482. doi:10.1016\/s1471-4922(02)02349-8.","DOI":"10.1016\/S1471-4922(02)02349-8"},{"key":"e_1_3_2_66_2","doi-asserted-by":"crossref","unstructured":"Bougdour A Durandau E Brenier-Pinchart MP Ortet P Barakat M Kieffer S Curt-Varesano A Curt-Bertini RL Bastien O Coute Y Pelloux H Hakimi MA. 2013. Host cell subversion by Toxoplasma GRA16 an exported dense granule protein that targets the host cell nucleus and alters gene expression. Cell Host Microbe 13(4):489\u2013500. doi:10.1016\/j.chom.2013.03.002.","DOI":"10.1016\/j.chom.2013.03.002"},{"key":"e_1_3_2_67_2","doi-asserted-by":"crossref","unstructured":"Okada T Marmansari D Li ZM Adilbish A Canko S Ueno A Shono H Furuoka H Igarashi M. 2013. A novel dense granule protein GRA22 is involved in regulating parasite egress in Toxoplasma gondii. Mol Biochem Parasitol 189(1-2):5\u201313. doi:10.1016\/j.molbiopara.2013.04.005.","DOI":"10.1016\/j.molbiopara.2013.04.005"},{"key":"e_1_3_2_68_2","doi-asserted-by":"crossref","unstructured":"Franco M Panas MW Marino ND Lee M-CW Buchholz KR Kelly FD Bednarski JJ Sleckman BP Pourmand N Boothroyd JC. 2016. A novel secreted protein MYR1 is central to Toxoplasma\u2019s manipulation of host cells. mBio 7(1):e02231-15. doi:10.1128\/mBio.02231-15.","DOI":"10.1128\/mBio.02231-15"},{"key":"e_1_3_2_69_2","doi-asserted-by":"crossref","unstructured":"Michelin A Bittame A Bordat Y Travier L Mercier C Dubremetz JF Lebrun M. 2009. GRA12 a Toxoplasma dense granule protein associated with the intravacuolar membranous nanotubular network. Int J Parasitol 39(3):299\u2013306. doi:10.1016\/j.ijpara.2008.07.011.","DOI":"10.1016\/j.ijpara.2008.07.011"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00260-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00260-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T22:56:30Z","timestamp":1646693790000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00260-21"}},"subtitle":[],"editor":[{"given":"Ira J.","family":"Blader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]},{"given":"Carolyn B.","family":"Coyne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,12,21]]},"references-count":68,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12,21]]}},"alternative-id":["10.1128\/mBio.00260-21"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00260-21","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.02.02.429490","asserted-by":"object"}]},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,21]]},"assertion":[{"value":"2021-02-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00260-21"}}