{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:53:55Z","timestamp":1792652035358,"version":"4.5.0"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2020,4,28]],"date-time":"2020-04-28T00:00:00Z","timestamp":1588032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,28]],"date-time":"2020-04-28T00:00:00Z","timestamp":1588032000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2020,4,28]]},"abstract":"<jats:p>\n                    Enterococci are opportunistic pathogens responsible for hospital- and community-acquired infections. All enterococci produce a surface polysaccharide called EPA (\n                    <jats:italic>e<\/jats:italic>\n                    nterococcal\n                    <jats:italic>p<\/jats:italic>\n                    olysaccharide\n                    <jats:italic>a<\/jats:italic>\n                    ntigen) required for biofilm formation, antibiotic resistance, and pathogenesis. Despite the critical role of EPA in cell growth and division and as a major virulence factor, no information is available on its structure. Here, we report the complete structure of the EPA polymer produced by the model strain\n                    <jats:named-content content-type=\"genus-species\">E. faecalis<\/jats:named-content>\n                    V583. We describe the structure of the EPA backbone, made of a rhamnan hexasaccharide substituted by Glc and GlcNAc residues, and show that teichoic acids are covalently bound to this rhamnan chain, forming the so-called \u201cEPA decorations\u201d essential for host colonization and pathogenesis. This report represents a key step in efforts to identify the structural properties of EPA that are essential for its biological activity and to identify novel targets to develop preventive and therapeutic approaches against enterococci.\n                  <\/jats:p>","DOI":"10.1128\/mbio.00277-20","type":"journal-article","created":{"date-parts":[[2020,4,27]],"date-time":"2020-04-27T13:13:34Z","timestamp":1587993214000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Complete Structure of the Enterococcal Polysaccharide Antigen (EPA) of Vancomycin-Resistant Enterococcus faecalis V583 Reveals that EPA Decorations Are Teichoic Acids Covalently Linked to a Rhamnopolysaccharide Backbone"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4967-9512","authenticated-orcid":false,"given":"Yann","family":"Guerardel","sequence":"first","affiliation":[{"name":"Univ. 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A hidden Markov model for predicting transmembrane helices in protein sequences. 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