{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T08:17:18Z","timestamp":1789633038196,"version":"build-2803163510"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T00:00:00Z","timestamp":1585008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,28]],"date-time":"2020-04-28T00:00:00Z","timestamp":1588032000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"MARION MERRELL DOW ENDOWMENT"},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["2R01AO58145"],"award-info":[{"award-number":["2R01AO58145"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI106925"],"award-info":[{"award-number":["R01AI106925"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009504","name":"University of Missouri-Kansas City","doi-asserted-by":"publisher","award":["unrestricted research funds"],"award-info":[{"award-number":["unrestricted research funds"]}],"id":[{"id":"10.13039\/100009504","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009504","name":"University of Missouri-Kansas City","doi-asserted-by":"publisher","award":["Women's Council Graduate Assistance Fund"],"award-info":[{"award-number":["Women's Council Graduate Assistance Fund"]}],"id":[{"id":"10.13039\/100009504","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005321","name":"American College of Clinical Pharmacy Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2020,4,28]]},"abstract":"<jats:p>\n            One mechanism behind drug resistance is altered export out of the cell. This work is a multifaceted analysis of membrane efflux transporters in the human fungal pathogen\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . Bioinformatics evidence infers that there is a relatively large number of genes in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            that encode ABC efflux transporters. However, very few of these transporters have been directly characterized and analyzed for their potential role in drug resistance.\nOur objective was to determine if these undercharacterized proteins function as efflux transporters and then to better define whether their efflux substrates include antifungal drugs used to treat fungal infections. We chose six\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            potential plasma membrane ABC transporter genes for analysis and found that all six genes produced functional transporter proteins. We used two fungal systems to look for correlations between transporter function and drug resistance. These transporters have the potential to produce drug-resistant phenotypes in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . Continued characterization of these and other transporters may assist in the development of efflux inhibitor drugs.\n          <\/jats:p>","DOI":"10.1128\/mbio.00338-20","type":"journal-article","created":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T12:54:46Z","timestamp":1585054486000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Characterization of the Efflux Capability and Substrate Specificity of Aspergillus fumigatus PDR5-like ABC Transporters Expressed in Saccharomyces cerevisiae"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7328-4057","authenticated-orcid":false,"given":"Brooke D.","family":"Esquivel","sequence":"first","affiliation":[{"name":"Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri\u2014Kansas City, Kansas City, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Rybak","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Translational Science, University of Tennessee Health Science Center, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine S.","family":"Barker","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Translational Science, University of Tennessee Health Science Center, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarrod R.","family":"Fortwendel","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Translational Science, University of Tennessee Health Science Center, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. David","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Translational Science, University of Tennessee Health Science Center, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore C.","family":"White","sequence":"additional","affiliation":[{"name":"Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri\u2014Kansas City, Kansas City, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2006.01.001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693780902717018"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1507.090043"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01292-08"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2016.00011"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2011.11.001"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00905-16"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-11-2743"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01343-10"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01095-08"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00051-08"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0924-8579(03)00213-9"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.12703\/P7-14"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2012.05.002"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03281.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02814.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-177"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.10.007"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.12.043"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12189"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092611-150111"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204707200"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99946-1"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00070"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt075"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1087-1845(02)00016-6"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00171-13"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-006-0073-2"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05003-14"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01320"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05706-11"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.4161\/bbug.1.6.13257"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2002.01516.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652015000700011"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/44.1.27"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(93)90081-W"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.105-116.2001"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.01.075"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00344-11"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.48.3.970-978.2004"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/88.11.699"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.29.17152"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.9.2.523"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2009.0872"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.10.110"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-5255-z"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780400029114"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0076-6879(02)50957-5"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.3791\/253"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320101310"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2013.06.004"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01252-16"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00437-19"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02478-15"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00854-09"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00103-08"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00338-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00338-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T02:52:17Z","timestamp":1646707937000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00338-20"}},"subtitle":[],"editor":[{"given":"Alexander","family":"Idnurm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,4,28]]},"references-count":56,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,4,28]]}},"alternative-id":["10.1128\/mBio.00338-20"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00338-20","relation":{},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,28]]},"assertion":[{"value":"2020-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00338-20"}}