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Plasmids are a central reason for the rapid global spread of bacterial resistance to antibiotics. In a single experimental procedure, this method replaces many traditional plasmid analysis techniques that together provide limited details and are slow to perform. The method is much faster than long-read whole-genome sequencing and offers direct genetic comparison of patient samples. We could conclude that no transfer of resistance plasmids had occurred between different bacteria during the outbreak and that secondary cases of ESBL-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            carriage were instead likely due to influx of new strains. We believe that the method offers potential in improving surveillance and infection control of resistant bacteria in hospitals.\n          <\/jats:p>","DOI":"10.1128\/mbio.00347-19","type":"journal-article","created":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T09:23:19Z","timestamp":1562577799000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Optical DNA Mapping Combined with Cas9-Targeted Resistance Gene Identification for Rapid Tracking of Resistance Plasmids in a Neonatal Intensive Care Unit Outbreak"],"prefix":"10.1128","volume":"10","author":[{"given":"Santosh K.","family":"Bikkarolla","sequence":"first","affiliation":[{"name":"Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viveka","family":"Nordberg","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Karolinska University Hospital, Stockholm, Sweden"},{"name":"Department of Clinical Science, Intervention and Technology, Division of Pediatrics, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4211-5696","authenticated-orcid":false,"given":"Fredrika","family":"Rajer","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4554-2124","authenticated-orcid":false,"given":"Vilhelm","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Humaun","family":"Kabir","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sriram","family":"KK","sequence":"additional","affiliation":[{"name":"Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albertas","family":"Dvirnas","sequence":"additional","affiliation":[{"name":"Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Ambj\u00f6rnsson","sequence":"additional","affiliation":[{"name":"Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian G.","family":"Giske","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Nav\u00e9r","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Karolinska University Hospital, Stockholm, Sweden"},{"name":"Department of Clinical Science, Intervention and Technology, Division of Pediatrics, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7382-9782","authenticated-orcid":false,"given":"Linus","family":"Sandegren","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4767-4868","authenticated-orcid":false,"given":"Fredrik","family":"Westerlund","sequence":"additional","affiliation":[{"name":"Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Tackling drug-resistant infections globally: final report and recommendations","author":"Review on Antimicrobial Resistance","year":"2016","unstructured":"Review on Antimicrobial Resistance. 2016. 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