{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:36:00Z","timestamp":1791311760790,"version":"4.3.1"},"reference-count":71,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2013,8,30]],"date-time":"2013-08-30T00:00:00Z","timestamp":1377820800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2013,8,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n                    causes the potentially fatal disease melioidosis. It is generally accepted that\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    is a noncommensal bacterium and that any culture-positive clinical specimen denotes disease requiring treatment. Over a 23-year study of melioidosis cases in Darwin, Australia, just one patient from 707 survivors has developed persistent asymptomatic\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    carriage. To better understand the mechanisms behind this unique scenario, we performed whole-genome analysis of two strains isolated 139\u00a0months apart. During this period,\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    underwent several adaptive changes. Of 23 point mutations, 78% were nonsynonymous and 43% were predicted to be deleterious to gene function, demonstrating a strong propensity for positive selection. Notably, a nonsense mutation inactivated the universal stress response sigma factor RpoS, with pleiotropic implications. The genome underwent substantial reduction, with four deletions in chromosome 2 resulting in the loss of 221 genes. The deleted loci included genes involved in secondary metabolism, environmental survival, and pathogenesis. Of 14 indels, 11 occurred in coding regions and 9 resulted in frameshift mutations that dramatically affected predicted gene products. Disproportionately, four indels affected lipopolysaccharide biosynthesis and modification. Finally, we identified a frameshift mutation in both P314 isolates within\n                    <jats:italic>wcbR<\/jats:italic>\n                    , an important component of the capsular polysaccharide I locus, suggesting virulence attenuation early in infection. Our study illustrates a unique clinical case that contrasts a high-consequence infectious agent with a long-term commensal infection and provides further insights into bacterial evolution within the human host.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    Some bacterial pathogens establish long-term infections that are difficult or impossible to eradicate with current treatments. Rapid advances in genome sequencing technologies provide a powerful tool for understanding bacterial persistence within the human host.\n                    <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n                    is considered a highly pathogenic bacterium because infection is commonly fatal. Here, we document within-host evolution of\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    in a unique case of human infection with ongoing chronic carriage. Genomic comparison of isolates obtained 139\u00a0months (11.5\u00a0years) apart showed a strong signal of adaptation within the human host, including inactivation of virulence and immunogenic factors, and deletion of pathways involved in environmental survival. Two global regulatory genes were mutated in the 139-month isolate, indicating extensive regulatory changes favoring bacterial persistence. Our study provides insights into\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    pathogenesis and, more broadly, identifies parallel evolutionary mechanisms that underlie chronic persistence of all bacterial pathogens.\n                  <\/jats:p>","DOI":"10.1128\/mbio.00388-13","type":"journal-article","created":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T22:12:18Z","timestamp":1374012738000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":111,"title":["Within-Host Evolution of Burkholderia pseudomallei over a Twelve-Year Chronic Carriage Infection"],"prefix":"10.1128","volume":"4","author":[{"given":"Erin P.","family":"Price","sequence":"first","affiliation":[{"name":"Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia"},{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek S.","family":"Sarovich","sequence":"additional","affiliation":[{"name":"Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia"},{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Mayo","sequence":"additional","affiliation":[{"name":"Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apichai","family":"Tuanyok","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin P.","family":"Drees","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjam","family":"Kaestli","sequence":"additional","affiliation":[{"name":"Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen M.","family":"Beckstrom-Sternberg","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James S.","family":"Babic-Sternberg","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"Kidd","sequence":"additional","affiliation":[{"name":"Queensland Children\u2019s Medical Research Institute, The University of Queensland, Herston, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott C.","family":"Bell","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, The Prince Charles Hospital, Chermside, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Keim","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talima","family":"Pearson","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart J.","family":"Currie","sequence":"additional","affiliation":[{"name":"Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia"},{"name":"Infectious Diseases Department and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1204699"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.383-416.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000900"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.997"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602138103"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01147-08"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024301"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.08.003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evq003"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(98)01312-2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/35059006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036507"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090110-102946"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.23.7008-7014.2003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1088"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00990.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2011.00327.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.4.2008-2017.2002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1111020108"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.7.2082-2087.2003"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01657-08"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030789"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00139"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01139.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00285-07"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001453"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01023-10"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.4.1324-1333.2005"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00501-0"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01218-10"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01262-05"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001068"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.3.465"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.3109\/00313027909061954"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2006.12.002"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000725"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/cfg.70"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403302101"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00440-06"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(08)70032-4"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1080\/07853890510037329"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.10.045"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015693"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028831"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.1150-1154.2004"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000327"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(00)90333-X"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.08.008"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-43-2-293"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05805-11"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.2.1106-1115.2005"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2000.0430"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1981.tb00494.x"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13374-0"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1676-1677.2001"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.58.12.1087"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1707.100614"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.1.85-90.2006"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-7-68"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1917404"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq268"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.074492.107"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011147"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.107524.110"},{"key":"e_1_3_2_68_2","doi-asserted-by":"crossref","unstructured":"LiH\nHandsakerB\nWysokerA\nFennellT\nRuanJ\nHomerN\nMarthG\nAbecasisG\nDurbinR 1000 Genome Project Data Processing Subgroup. 2009. The sequence alignment\/map format and SAMtools. Bioinformatics 25:2078\u20132079.","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq033"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp666"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.4161\/fly.19695"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046688"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00388-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00388-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T20:51:01Z","timestamp":1646686261000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00388-13"}},"subtitle":[],"editor":[{"given":"Jeff F.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,8,30]]},"references-count":71,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,8,30]]}},"alternative-id":["10.1128\/mBio.00388-13"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00388-13","relation":{},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,30]]},"assertion":[{"value":"2013-05-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00388-13"}}