{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:13:39Z","timestamp":1790669619292,"version":"4.1.0"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T00:00:00Z","timestamp":1585008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,28]],"date-time":"2020-04-28T00:00:00Z","timestamp":1588032000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["31003A_153200"],"award-info":[{"award-number":["31003A_153200"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["31003A_175557"],"award-info":[{"award-number":["31003A_175557"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]},{"name":"OPO foundation"},{"DOI":"10.13039\/100008273","name":"Novartis Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008273","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-62-IBEID"],"award-info":[{"award-number":["ANR-10-LABX-62-IBEID"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2020,4,28]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            is a ubiquitous environmental bacterium which, upon inhalation, causes a life-threatening pneumonia termed Legionnaires\u2019 disease. The opportunistic pathogen grows in amoebae and macrophages by employing a \u201ctype IV\u201d secretion system, which secretes more than 300 different \u201ceffector\u201d proteins into the host cell, where they subvert pivotal processes. The function of many of these effector proteins is unknown, and their evolution has not been studied.\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            RCC1 repeat effectors target the small GTPase Ran, a molecular switch implicated in different cellular processes such as nucleocytoplasmic transport and microtubule cytoskeleton dynamics. We provide evidence that one or more RCC1 repeat genes are distributed in two main clusters of\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            strains and have divergently evolved to target different components of the Ran GTPase activation cycle at different subcellular sites. Thus,\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            employs a sophisticated strategy to subvert host cell Ran GTPase during infection.\n          <\/jats:p>","DOI":"10.1128\/mbio.00405-20","type":"journal-article","created":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T12:54:46Z","timestamp":1585054486000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Divergent Evolution of\n            <i>Legionella<\/i>\n            RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets"],"prefix":"10.1128","volume":"11","author":[{"given":"A. Leoni","family":"Swart","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Steiner","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Gomez-Valero","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Bact\u00e9ries Intracellulaires, Paris, France"},{"name":"CNRS UMR 3525, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabina","family":"Sch\u00fctz","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandy","family":"Hannemann","sequence":"additional","affiliation":[{"name":"Center for Integrated Protein Science Munich, Department of Chemistry, Technical University Munich, Garching, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Janning","sequence":"additional","affiliation":[{"name":"Max Planck Institut f\u00fcr Molekulare Physiologie, Dortmund, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Irminger","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Rothmeier","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute, Ludwig-Maximilians University Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Buchrieser","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Bact\u00e9ries Intracellulaires, Paris, France"},{"name":"CNRS UMR 3525, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aymelt","family":"Itzen","sequence":"additional","affiliation":[{"name":"Center for Integrated Protein Science Munich, Department of Chemistry, Technical University Munich, Garching, Germany"},{"name":"Institute for Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikram Govind","family":"Panse","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5462-9301","authenticated-orcid":false,"given":"Hubert","family":"Hilbi","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100913-013439"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2015.1"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00061"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00052-09"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.08.010"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12450"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.67"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1967"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00482"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2017.08.004"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/82_2013_341"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020046"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00839-13"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02420-18"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01219.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807505200"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1207903109"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.236"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00025"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1522067113"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1463"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914231107"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2010.97"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1192276"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/82_2013_340"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.06.001"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01512-5"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1715361115"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1717383115"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.02.003"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601562103"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001289"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01301.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2013.86"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.03.004"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00851.x"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12256"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M116.063453"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03071"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.015289"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2114"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0340716"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2410"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.04.043"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709397200"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/354080a0"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003598"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1447"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.22.7716-7726.2005"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00208"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000278"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.170746"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12258"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.07.020"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/32204"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501315102"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01249.x"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002988"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.9.7.2989"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201743903"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1243"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0524-x"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1224503"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01005.x"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00158-18"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7493-1_7"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00405-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00405-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T03:00:19Z","timestamp":1646708419000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00405-20"}},"subtitle":[],"editor":[{"given":"Craig R.","family":"Roy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,4,28]]},"references-count":67,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,4,28]]}},"alternative-id":["10.1128\/mBio.00405-20"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00405-20","relation":{},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,28]]},"assertion":[{"value":"2020-02-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00405-20"}}