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Here, we have developed an engineered 3D human brain microvessel model that mimics the blood flow rates and architecture of small blood vessels to study how\n                    <jats:named-content content-type=\"genus-species\">P. falciparum<\/jats:named-content>\n                    <jats:italic>-<\/jats:italic>\n                    infected human erythrocytes attach to brain endothelial cells. By studying parasite lines with different adhesive properties, we show that the malaria parasite binding rate is heterogeneous and strongly influenced by physiological differences in flow and whether the endothelium has been previously activated by TNF-\u03b1, a proinflammatory cytokine that is linked to malaria disease severity. We also show the importance of human EPCR and ICAM-1 in parasite binding. Our model sheds new light on how\n                    <jats:named-content content-type=\"genus-species\">P. falciparum<\/jats:named-content>\n                    binds within brain microvessels and provides a powerful method for future investigations of recruitment of human brain pathogens to the blood vessel lining of the brain.\n                  <\/jats:p>","DOI":"10.1128\/mbio.00420-19","type":"journal-article","created":{"date-parts":[[2019,5,25]],"date-time":"2019-05-25T06:37:54Z","timestamp":1558766274000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Binding Heterogeneity of Plasmodium falciparum to Engineered 3D Brain Microvessels Is Mediated by EPCR and ICAM-1"],"prefix":"10.1128","volume":"10","author":[{"given":"Maria","family":"Bernabeu","sequence":"first","affiliation":[{"name":"Seattle Children\u2019s Research Institute, Seattle, Washington, 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