{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:17:58Z","timestamp":1790713078647,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T00:00:00Z","timestamp":1686182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T00:00:00Z","timestamp":1686182400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"MOST | National Key Research and Development Program of China","award":["2020YFA0907200,2018YFA0901203"],"award-info":[{"award-number":["2020YFA0907200,2018YFA0901203"]}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32030001"],"award-info":[{"award-number":["32030001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Acidovorax citrulli<\/jats:italic>\n            is a gram-negative plant pathogen that employs the type \u2162 secretion system (T3SS) to infect cucurbit crops and cause bacterial fruit blotch. This bacterium also possesses an active type \u2165 secretion system (T6SS) with strong antibacterial and antifungal activities. However, how plant cells respond to these two secretion systems and whether there is any cross talk between T3SS and T6SS during infection remain unknown. Here, we employ transcriptomic analysis to compare cellular responses to the T3SS and the T6SS during\n            <jats:italic>in planta<\/jats:italic>\n            infection and report distinctive effects on multiple pathways. The T3SS-mediated differentially expressed genes were enriched in the pathways of phenylpropanoid biosynthesis, plant-pathogen interaction, MAPK signaling pathway, and glutathione metabolism, while the T6SS uniquely affected genes were related to photosynthesis. The T6SS does not contribute to the\n            <jats:italic>in planta<\/jats:italic>\n            virulence of\n            <jats:italic>A. citrulli<\/jats:italic>\n            but is critical for the survival of the bacterium when mixed with watermelon phyllosphere bacteria. In addition, T3SS-mediated virulence is independent of the T6SS, and the inactivation of the T3SS does not affect the T6SS-mediated competition against a diverse set of bacterial pathogens that commonly contaminate edible plants or directly infect plants. A T6SS-active T3SS-null mutant (Ac\n            <jats:sub>av<\/jats:sub>\n            ) could inhibit the growth of\n            <jats:italic>Xanthomonas oryzae<\/jats:italic>\n            pv.\n            <jats:italic>oryzae<\/jats:italic>\n            significantly both\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            and also reduce symptoms of rice bacterial blight. In conclusion, our data demonstrate the T6SS in\n            <jats:italic>A. citrulli<\/jats:italic>\n            is nonpathogenic to the plant host and can be harnessed as a pathogen killer against plant-associated bacteria.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Chemical pesticides are widely used to protect crops from various pathogens. Still, their extensive use has led to severe consequences, including drug resistance and environmental contamination. Here, we show that an engineered T6SS-active, but avirulent mutant of\n              <jats:italic>Acidovorax citrulli<\/jats:italic>\n              has strong inhibition capabilities against several pathogenic bacteria, demonstrating an effective strategy that is an alternative to chemical pesticides for sustainable agricultural practices.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.00459-23","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T13:00:16Z","timestamp":1686229216000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Differential plant cell responses to\n            <i>Acidovorax citrulli<\/i>\n            T3SS and T6SS reveal an effective strategy for controlling plant-associated pathogens"],"prefix":"10.1128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5032-9507","authenticated-orcid":true,"given":"Yumin","family":"Kan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3557-1850","authenticated-orcid":true,"given":"Tao","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, Guangdong, 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