{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T17:32:45Z","timestamp":1770744765464,"version":"3.49.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Scientific Society University of Freiburg, Germany","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2023,10,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Recent studies have linked proton pump inhibitor (PPI) treatment to increased complications of cirrhosis, such as bacterial infections and hepatic encephalopathy. However, the underlying pathophysiological mechanisms remain unclear. The present study investigated the hypothesis that PPI treatment may promote adverse effects in patients with advanced cirrhosis by affecting subclinical bacterial translocation (BT) from the gut into the portal venous bloodstream. Blood samples from the portal vein were obtained during implantation of a transjugular intrahepatic portosystemic shunt (TIPS) in a total of 80 cirrhosis patients with PPI treatment (PPI group,\n            <jats:italic>n<\/jats:italic>\n            = 57) and without PPI treatment (no-PPI group,\n            <jats:italic>n<\/jats:italic>\n            = 23). BT was identified using a 16S ribosomal RNA gene (V1V2 region) polymerase chain reaction. The microbiota composition in the portal venous blood samples was further analyzed by deep amplicon sequencing. Indeed, the prevalence of BT was significantly higher in the PPI group compared to the no-PPI group (86.0% vs 52.2%,\n            <jats:italic>P<\/jats:italic>\n            = 0.001). Importantly, this effect was not attributable to group differences in the severity of cirrhosis, parameters of portal hypertension, or medication. Microbiome analyses showed significantly increased alpha-diversity (Shannon) in the portal venous blood of the PPI group. Taxonomic analyses revealed significantly increased\n            <jats:italic>Streptococcus<\/jats:italic>\n            abundances in these patients. The present study reveals aggravated BT in patients with advanced cirrhosis and portal hypertension receiving PPI therapy. Increased BT could be an important pathomechanism contributing to the adverse effects of PPI treatment in patients with cirrhosis.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>Long-term prescription of proton pump inhibitors (PPIs) in patients with cirrhosis is common practice. However, in recent years, several observational studies have reported increased complications and negative prognostic effects of PPI treatment in these patients. Judging the significance of these associations is complicated by the fact that a plausible underlying pathomechanism has not been identified so far. In the present study, we address this important issue by investigating the impact of PPI treatment on subclinical bacterial translocation from the gut into the blood stream in patients with advanced cirrhosis and portal hypertension. Indeed, we report significantly aggravated bacterial translocation in cirrhosis patients receiving PPI treatment. This finding is highly relevant, as bacterial translocation is known to promote the development of complications and impair prognosis in patients with cirrhosis. Hence, the present study could establish a plausible link between PPI treatment and adverse effects in cirrhosis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.00492-23","type":"journal-article","created":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T13:01:28Z","timestamp":1692968488000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Proton pump inhibitor treatment aggravates bacterial translocation in patients with advanced cirrhosis and portal hypertension"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3623-7857","authenticated-orcid":false,"given":"Lukas","family":"Sturm","sequence":"first","affiliation":[{"name":"Department of Medicine II, Faculty of Medicine, Medical Center University of Freiburg , Freiburg, Germany"},{"name":"Berta-Ottenstein-Programme, Faculty of Medicine, University of Freiburg , Freiburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Misa","family":"Hirose","sequence":"additional","affiliation":[{"name":"Luebeck Institute of Experimental Dermatology, University of Luebeck , Luebeck, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Stolz","sequence":"additional","affiliation":[{"name":"Department of Medicine II, Faculty of Medicine, Medical Center University of Freiburg , Freiburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Schultheiss","sequence":"additional","affiliation":[{"name":"Department of Medicine II, Faculty of Medicine, Medical Center University of Freiburg , Freiburg, Germany"},{"name":"Berta-Ottenstein-Programme, Faculty of Medicine, University of Freiburg , Freiburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Zoldan","sequence":"additional","affiliation":[{"name":"Department of Medicine II, 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