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They are autochthonous to aquatic environments globally, but traditional metagenomic methods for detecting and typing pathogenic\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      spp. are challenged by their presence in relatively low abundance and ability to persist in a viable but nonculturable state. In the study reported here, hybridization capture sequencing (HCS) was employed to profile low-abundance\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      spp. in environmental samples. The HCS panel targeted a family of molecular chaperones (CPN60) specific to 69\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      spp. and 162\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      -specific virulence factors. This approach was evaluated in parallel with traditional whole-community shotgun sequencing in a metagenomic analysis of water and oyster samples collected from the Chesapeake Bay. In addition,\n                      <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Vibrio vulnificus<\/jats:italic>\n                      strains isolated from the samples were subjected to whole-genome sequencing to determine the genetic characteristics of pathogenic\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      spp. circulating in an aquatic environment. HCS, employed to determine the incidence and characterization of specific\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      spp., yielded significantly greater metagenomic insight, notably a variety of other\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      spp., including detection of\n                      <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Vibrio fluvialis<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Vibrio aestuarianus<\/jats:italic>\n                      , in addition to\n                      <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Vibrio vulnificus<\/jats:italic>\n                      , and also important virulence factors not detectable using traditional molecular methods. Thus, pathogenic\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      spp. in aquatic ecosystems may be far more common than currently understood. It is concluded that environmental surveillance should include HCS, a valuable tool for the detection and characterization of pathogenic agents in aquatic ecosystems, notably vibrios.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        The increasing prevalence of pathogenic\n                        <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                        spp. in aquatic ecosystems, driven by climate change, is closely linked to a rise in cholera and vibriosis cases, emphasizing the need for improved environmental surveillance. Vibrios are naturally occurring in aquatic environments globally, but traditional metagenomic methods for detecting and typing pathogenic\n                        <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                        spp. are challenged by their presence in relatively low abundance and ability to persist in a viable but nonculturable state. In the study reported here, hybridization capture sequencing was employed to profile low-abundance\n                        <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                        spp. in metagenomic samples, namely water and oysters collected from the Chesapeake Bay. This approach was evaluated in parallel with traditional whole-community shotgun sequencing and whole-genome sequencing of\n                        <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                        and\n                        <jats:italic toggle=\"yes\">Vibrio vulnificus<\/jats:italic>\n                        strains isolated from the samples. Results suggest pathogenic\n                        <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                        spp. in aquatic ecosystems may be far more common than currently understood, when multiple methods are considered for environmental surveillance.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.00516-25","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T09:02:28Z","timestamp":1750842148000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Hybridization capture sequencing for\n                    <i>Vibrio<\/i>\n                    spp. and associated virulence factors"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3234-3337","authenticated-orcid":true,"given":"Kyle 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