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Concomitant pneumococcal and influenza vaccination improves protection against coinfection but does not always yield complete protection. Impaired innate and adaptive immune responses have been associated with attenuated bacterial clearance in influenza virus-infected hosts. In this study, we showed that preceding low-dose IAV infection caused persistent\n                    <jats:italic>Sp<\/jats:italic>\n                    infection and suppression of bacteria-specific T-helper type 17 (Th17) responses in mice. Prior\n                    <jats:italic>Sp<\/jats:italic>\n                    infection protected against subsequent IAV\/\n                    <jats:italic>Sp<\/jats:italic>\n                    coinfection by improving bacterial clearance and rescuing bacteria-specific Th17 responses in the lungs. Furthermore, blockade of IL-17A by anti-IL-17A antibodies abrogated the protective effect of\n                    <jats:italic>Sp<\/jats:italic>\n                    preinfection. Importantly, memory Th17 responses induced by\n                    <jats:italic>Sp<\/jats:italic>\n                    preinfection overcame viral-driven Th17 inhibition and provided cross-protection against different\n                    <jats:italic>Sp<\/jats:italic>\n                    serotypes following coinfection with IAV. These results indicate that bacteria-specific Th17 memory cells play a key role in providing protection against IAV\/\n                    <jats:italic>Sp<\/jats:italic>\n                    coinfection in a serotype-independent manner and suggest that a Th17-based vaccine would have excellent potential to mitigate disease caused by coinfection.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                      (\n                      <jats:italic>Sp<\/jats:italic>\n                      ) frequently causes secondary bacterial pneumonia after influenza A virus (IAV) infection, leading to increased morbidity and mortality worldwide. Current pneumococcal vaccines induce highly strain-specific antibody responses and provide limited protection against IAV\/\n                      <jats:italic>Sp<\/jats:italic>\n                      coinfection. Th17 responses are broadly protective against\n                      <jats:italic>Sp<\/jats:italic>\n                      single infection, but whether the Th17 response, which is dramatically impaired by IAV infection in na\u00efve mice, might be effective in immunization-induced protection against pneumonia caused by coinfection is not known. In this study, we have revealed that\n                      <jats:italic>Sp<\/jats:italic>\n                      -specific memory Th17 cells rescue IAV-driven inhibition and provide cross-protection against subsequent lethal coinfection with IAV and different\n                      <jats:italic>Sp<\/jats:italic>\n                      serotypes. These results indicate that a Th17-based vaccine would have excellent potential to mitigate disease caused by IAV\/\n                      <jats:italic>Sp<\/jats:italic>\n                      coinfection.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.00519-23","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T09:00:38Z","timestamp":1684918838000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Memory Th17 cell-mediated protection against lethal secondary pneumococcal pneumonia following influenza infection"],"prefix":"10.1128","author":[{"given":"Yong","family":"Li","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"},{"name":"Shanghai Institute of Immunology, Shanghai Jiaotong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dafan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"},{"name":"Shanghai Sixth People\u2019s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"},{"name":"Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengsen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"},{"name":"Department of Endoscopy, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Cancer and Basic Medicine (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandy M.","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengwen","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"},{"name":"Department of Emergency Medical, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 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