{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:14:47Z","timestamp":1790291687407,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EY028320"],"award-info":[{"award-number":["EY028320"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI172335"],"award-info":[{"award-number":["AI172335"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI106699"],"award-info":[{"award-number":["AI106699"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI106699"],"award-info":[{"award-number":["AI106699"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,6,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The opportunistic pathogen\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      causes debilitating lung infections in people with cystic fibrosis, as well as eye, burn, and wound infections in otherwise immunocompetent individuals. Many of\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      \u2019s virulence factors are regulated by environmental cues, such as temperature and cell density. One such virulence factor is protease IV. Prior studies have shown that\n                      <jats:italic toggle=\"yes\">piv<\/jats:italic>\n                      expression is higher at ambient temperatures (22\u00b0C\u201328\u00b0C) compared to human body temperature (37\u00b0C) and also upregulated by the LasRI quorum sensing system, although it is unclear how. We found that\n                      <jats:italic toggle=\"yes\">piv<\/jats:italic>\n                      expression was thermoregulated at stationary phase, but not exponential phase, and that\n                      <jats:italic toggle=\"yes\">piv<\/jats:italic>\n                      is thermoregulated at the level of transcription. Using a transcriptional reporter for\n                      <jats:italic toggle=\"yes\">piv<\/jats:italic>\n                      , we show that LasR activates\n                      <jats:italic toggle=\"yes\">piv<\/jats:italic>\n                      expression more at 25\u00b0C at stationary phase than at 37\u00b0C. We show that key components of the LasRI quorum sensing system are not upregulated at 25\u00b0C, suggesting that LasR regulatory activity is not higher intrinsically at this temperature. We also identified sequences within the\n                      <jats:italic toggle=\"yes\">piv<\/jats:italic>\n                      promoter that are important for its thermoregulation. We propose that LasR upregulates\n                      <jats:italic toggle=\"yes\">piv<\/jats:italic>\n                      more at 25\u00b0C than at 37\u00b0C. The finding that temperature controls LasR regulation of\n                      <jats:italic toggle=\"yes\">piv<\/jats:italic>\n                      highlights the complex nature of gene regulatory systems in\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      .\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                        is a versatile opportunistic pathogen capable of causing many different types of infections that are often difficult to treat, such as lung infections in people with cystic fibrosis. Temperature regulates the expression of many virulence factors that contribute to\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        \u2019s ability to cause infection, yet our mechanistic understanding of virulence factor thermoregulation is poor. In this study, we show that the virulence factor protease IV is thermoregulated at the level of transcription through the quorum sensing regulator, LasR. Mechanistic studies of virulence factor thermoregulation will expand our understanding of how\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        experiences different environments, including the mammalian host. Our work also highlights the importance of growth conditions in studying gene regulation, as it better elucidates the regulation of protease IV by LasR, which was previously not well understood.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.00541-25","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T09:03:36Z","timestamp":1747731816000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Temperature controls LasR regulation of\n                    <i>piv<\/i>\n                    expression in\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7493-942X","authenticated-orcid":false,"given":"Rachel 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