{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:36:21Z","timestamp":1792665381067,"version":"4.5.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T00:00:00Z","timestamp":1383609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2013,12,31]],"date-time":"2013-12-31T00:00:00Z","timestamp":1388448000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2013,12,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We constructed a near-saturation transposon mutant library for\n                    <jats:named-content content-type=\"genus-species\">Burkholderia thailandensis<\/jats:named-content>\n                    , a low-virulence surrogate for the causative agent of melioidosis (\n                    <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n                    ). A primary set of nearly 42,000 unique mutants (~7.5 mutants\/gene) was generated using transposon Tn\n                    <jats:italic>5<\/jats:italic>\n                    derivatives. The strains carry insertions in 87% of the predicted protein-coding genes of the organism, corresponding to nearly all of those nonessential for growth on nutrient agar. To achieve high genome coverage, we developed procedures for efficient sequence identification of insertions in extremely GC-rich regions of DNA. To facilitate strain distribution, we created a secondary library with two mutants per gene for which most transposon locations had been confirmed by resequencing. A map of mutations in the two-allele library and procedures for obtaining strains can be found at\n                    <jats:uri xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/tools.nwrce.org\/tn_mutants\/\" xlink:type=\"simple\">http:\/\/tools.nwrce.org\/tn_mutants\/<\/jats:uri>\n                    and\n                    <jats:uri xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.gs.washington.edu\/labs\/manoil\/\" xlink:type=\"simple\">http:\/\/www.gs.washington.edu\/labs\/manoil\/<\/jats:uri>\n                    . The library should facilitate comprehensive mutant screens and serve as a source of strains to test predicted genotype-phenotype associations.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    The Gram-negative bacterium\n                    <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n                    is a biothreat agent due to its potential for aerosol delivery and intrinsic antibiotic resistance and because exposure produces pernicious infections. Large-scale studies of\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    are limited by the fact that the organism must be manipulated under biological safety level 3 conditions. A close relative of\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    called\n                    <jats:named-content content-type=\"genus-species\">Burkholderia thailandensis<\/jats:named-content>\n                    , which can be studied under less restrictive conditions, has been validated as a low-virulence surrogate in studies of virulence, antibiotic resistance and other traits. To facilitate large-scale studies of\n                    <jats:named-content content-type=\"genus-species\">B. thailandensis<\/jats:named-content>\n                    , we created a near-saturation, sequence-defined transposon mutant library of the organism. The library facilitates genetic studies that identify genotype-phenotype associations conserved in\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/mbio.00604-13","type":"journal-article","created":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T22:07:47Z","timestamp":1383689267000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":84,"title":["Sequence-Defined Transposon Mutant Library of Burkholderia thailandensis"],"prefix":"10.1128","volume":"4","author":[{"given":"Larry A.","family":"Gallagher","sequence":"first","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Ramage","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapatbhorn","family":"Patrapuvich","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eli","family":"Weiss","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitch","family":"Brittnacher","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Manoil","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1204699"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-1-317"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-46"},{"key":"e_1_3_2_5_2","first-page":"81","article-title":"Inflammation patterns induced by different Burkholderia species in mice","volume":"10","author":"Wiersinga WJ","year":"2008","unstructured":"WiersingaWJ de VosAF de BeerR WielandCW RoelofsJJ WoodsDE van der PollT . 2008. 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