{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:35:40Z","timestamp":1789130140143,"version":"build-2803163510"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T00:00:00Z","timestamp":1555977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI102584"],"award-info":[{"award-number":["R01AI102584"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,4,30]]},"abstract":"<jats:p>\n            Cyclic diguanylate monophosphate (c-di-GMP) is a broadly conserved bacterial signaling molecule that affects motility, biofilm formation, and virulence. Although it has been known that high intracellular concentrations of c-di-GMP correlate with motility suppression and biofilm formation, how the 53 predicted c-di-GMP modulators in\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            collectively influence motility is not understood in detail. Here we used a combination of plate assays and single-cell tracking methods to correlate motility and biofilm formation outcomes with specific enzymes involved in c-di-GMP synthesis in\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            , the causative agent of the disease cholera.\n          <\/jats:p>","DOI":"10.1128\/mbio.00670-19","type":"journal-article","created":{"date-parts":[[2019,4,22]],"date-time":"2019-04-22T11:33:20Z","timestamp":1555932800000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Functional Specialization in\n            <i>Vibrio cholerae<\/i>\n            Diguanylate Cyclases: Distinct Modes of Motility Suppression and c-di-GMP Production"],"prefix":"10.1128","volume":"10","author":[{"given":"David","family":"Zamorano-S\u00e1nchez","sequence":"first","affiliation":[{"name":"Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wujing","family":"Xian","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California, Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Calvin K.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California, Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Salinas","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiriya","family":"Thongsomboon","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynette","family":"Cegelski","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard C. L.","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California, Los Angeles, Los Angeles, California, USA"},{"name":"Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA"},{"name":"California Nano Systems Institute, University of California, Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fitnat H.","family":"Yildiz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.94"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3433"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00043-12"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2017.01.002"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090816-093325"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01639-17"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.VMBF-0015-2015"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601277103"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01624.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5913"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.3.1049-1059.2006"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01569-06"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1222981"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005068"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005232"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12481"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12432"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.5.1716-1726.2001"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1181185"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04155.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.10.3600-3613.2006"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-014-0272-9"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05106.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05568.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00564-08"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00209-10"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1115663109"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002719"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.435-445.2001"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(79)90280-0"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)84073-2"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)81047-2"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.060022997"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.24.11388"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.18.5097-5104.2000"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01770-12"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00589-0"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501259200"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.018"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.03.001"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07179.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep20871"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12799"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.040196-0"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-10-2395"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0030154.eor"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.475808"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01092-12"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00373-13"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1716231114"},{"key":"e_1_3_3_52_2","first-page":"825","article-title":"PilZ domain proteins bind cyclic diguanylate and regulate diverse processes in Vibrio cholerae","volume":"148","author":"Pratt JT","year":"2008","unstructured":"Pratt JT, Tamayo R, Tischler AD, Camilli A. 2008. PilZ domain proteins bind cyclic diguanylate and regulate diverse processes in Vibrio cholerae. J Biol Chem 148:825\u2013832.","journal-title":"J Biol Chem"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601918"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.324012"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01579"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02868-05"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051241"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00736-17"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00290-09"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1421082112"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004933"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1720071115"}],"updated-by":[{"DOI":"10.1128\/mbio.01960-20","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T00:00:00Z","timestamp":1600732800000}}],"container-title":["mBio"],"original-title":[],"contributor":[{"given":"Karen","family":"Visick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]},{"given":"Mark","family":"Schembri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00670-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00670-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T02:27:50Z","timestamp":1646706470000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00670-19"}},"subtitle":[],"editor":[{"given":"E.","family":"Peter Greenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,4,30]]},"references-count":61,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,4,30]]}},"alternative-id":["10.1128\/mBio.00670-19"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00670-19","relation":{"correction":[{"id-type":"doi","id":"10.1128\/mBio.01960-20","asserted-by":"object"}]},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,30]]},"assertion":[{"value":"2019-03-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00670-19"}}