{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T13:43:32Z","timestamp":1782222212621,"version":"3.54.5"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T00:00:00Z","timestamp":1559001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T00:00:00Z","timestamp":1561420800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31270170"],"award-info":[{"award-number":["31270170"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["3133002"],"award-info":[{"award-number":["3133002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31470236"],"award-info":[{"award-number":["31470236"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2015CB150600"],"award-info":[{"award-number":["2015CB150600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,6,25]]},"abstract":"<jats:p>\n            Zeamines are a family of newly identified phytotoxins and potent antibiotics produced by\n            <jats:italic>D. zeae<\/jats:italic>\n            EC1. Unlike most bacterial organisms, which are highly sensitive,\n            <jats:italic>D. zeae<\/jats:italic>\n            EC1 is tolerant to zeamines, but the mechanisms involved are unknown. Our study showed, for the first time, that a new RND efflux pump, DesABC, is indispensable for\n            <jats:italic>D. zeae<\/jats:italic>\n            EC1 against zeamines. We found that the DesABC efflux pump was zeamine specific and appeared to be conserved only in the\n            <jats:italic>Dickeya<\/jats:italic>\n            species, which may explain the high potency of zeamines against a wide range of bacterial pathogens. We also showed that expression of DesABC efflux system genes was induced by zeamines. These findings not only provide an answer to why\n            <jats:italic>D. zeae<\/jats:italic>\n            EC1 is much more tolerant to zeamines than other bacterial pathogens but also document a signaling role of zeamines in modulation of gene expression.\n          <\/jats:p>","DOI":"10.1128\/mbio.00713-19","type":"journal-article","created":{"date-parts":[[2019,5,25]],"date-time":"2019-05-25T10:37:54Z","timestamp":1558780674000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["A Substrate-Activated Efflux Pump, DesABC, Confers Zeamine Resistance to Dickeya zeae"],"prefix":"10.1128","volume":"10","author":[{"given":"Zhibin","family":"Liang","sequence":"first","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China"},{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luhao","family":"Huang","sequence":"additional","affiliation":[{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"He","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China"},{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China"},{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zurong","family":"Shi","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China"},{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China"},{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China"},{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfa","family":"Lv","sequence":"additional","affiliation":[{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Chen","sequence":"additional","affiliation":[{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4298-7988","authenticated-orcid":false,"given":"Lian-Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China"},{"name":"Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01472-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12073"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12376"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-04-11-0087"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-04-13-0098-R"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054143"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/c4sc01927j"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00137"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1039\/b916307g"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep15719"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03146-14"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1545-x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00117-14"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00327"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00587"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00660"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00007"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10641"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.9.5056-5065.2005"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00040-09"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-14-185"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.22.6487"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1114944109"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01068-14"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.22.7363-7372.1993"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.23.6490-6499.2002"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.7.2124-2131.2002"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3375-3380.2001"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116047"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00305"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01388-10"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2009.0320"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0399"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv362"},{"key":"e_1_3_2_36_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically","author":"Clinical and Laboratory Standards Institute","year":"2012","unstructured":"Clinical and Laboratory Standards Institute. 2012. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. CLSI document M07-A9. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx281"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01530-12"},{"key":"e_1_3_2_43_2","first-page":"61","volume-title":"Metagenomics and its applications in agriculture, biomedicine and environmental studies","author":"Solovyev V","year":"2011","unstructured":"Solovyev V, Salamov A. 2011. Automatic annotation of microbial genomes and metagenomic sequences, p 61\u201378. In Li RW (ed), Metagenomics and its applications in agriculture, biomedicine and environmental studies. Nova Science Publishers, Hauppage, NY."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2000.13.11.1243"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12415"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-18578-2"}],"container-title":["mBio"],"original-title":[],"contributor":[{"given":"Martin","family":"Welch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]},{"given":"Vittorio","family":"Venturi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00713-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00713-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T02:27:34Z","timestamp":1646706454000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00713-19"}},"subtitle":[],"editor":[{"given":"Susan","family":"Gottesman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,6,25]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,6,25]]}},"alternative-id":["10.1128\/mBio.00713-19"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00713-19","relation":{},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,25]]},"assertion":[{"value":"2019-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00713-19"}}