{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:27:20Z","timestamp":1792787240915,"version":"4.5.0"},"reference-count":157,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Rudolph Hugh Endowment, Michigan State","id":[{"id":"https:\/\/ror.org\/0229evh14","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Integrated Pharmacological Sciences Training Program","award":["2T32 GM092715"],"award-info":[{"award-number":["2T32 GM092715"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2024,8,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                      is a Gram-negative gastrointestinal pathogen responsible for the diarrheal disease cholera. Expression of key virulence factors, cholera toxin and toxin-coregulated pilus, is regulated directly by ToxT and indirectly by two transmembrane transcription regulators (TTRs), ToxR and TcpP, that promote the expression of\n                      <jats:italic toggle=\"yes\">toxT<\/jats:italic>\n                      . TcpP abundance and activity are controlled by TcpH, a single-pass transmembrane protein, which protects TcpP from a two-step proteolytic process known as regulated intramembrane proteolysis (RIP). The mechanism of TcpH-mediated protection of TcpP represents a major gap in our understanding of\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      pathogenesis. The absence of\n                      <jats:italic toggle=\"yes\">tcpH<\/jats:italic>\n                      leads to unimpeded degradation of TcpP\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and a colonization defect in a neonate mouse model of\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      colonization. Here, we show that TcpH protects TcpP from RIP\n                      <jats:italic toggle=\"yes\">via<\/jats:italic>\n                      direct interaction. We also demonstrate that \u03b1-linolenic acid, a dietary fatty acid, promotes TcpH-dependent inhibition of RIP\n                      <jats:italic toggle=\"yes\">via<\/jats:italic>\n                      co-association of TcpP and TcpH molecules within detergent-resistant membranes (DRMs) in a mechanism requiring the TcpH transmembrane domain. Taken together, our data support a model where\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      cells use exogenous \u03b1-linolenic acid to remodel the phospholipid bilayer\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      , leading to co-association of TcpP and TcpH within DRMs where RIP of TcpP is inhibited by TcpH, thereby promoting\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      pathogenicity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                      continues to pose a significant global burden on health and an alternative therapeutic approach is needed, due to evolving multidrug resistance strains. Transcription of\n                      <jats:italic toggle=\"yes\">toxT<\/jats:italic>\n                      , stimulated by TcpP and ToxR, is essential for\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      pathogenesis. Our results show that TcpP, one of the major regulators of\n                      <jats:italic toggle=\"yes\">toxT<\/jats:italic>\n                      gene expression, is protected from proteolysis by TcpH,\n                      <jats:italic toggle=\"yes\">via<\/jats:italic>\n                      direct interaction. Furthermore, we identified a gut metabolite, \u03b1-linolenic acid, that stimulates the co-association of TcpP and TcpH within detergent-resistant membranes (also known as lipid-ordered membrane domains), thereby supporting TcpH-dependent antagonism of TcpP proteolysis. Data presented here extend our knowledge of RIP, virulence gene regulation in\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      , and, to the best of our knowledge, provides the first evidence that lipid-ordered membranes exist within\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      . The model presented here also suggests that TTRs, common among bacteria and archaea, and co-component signal transduction systems present in\n                      <jats:italic toggle=\"yes\">Enterobacteria<\/jats:italic>\n                      , could also be influenced similarly.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.00721-24","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T08:58:22Z","timestamp":1719997102000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["An essential host dietary fatty acid promotes TcpH inhibition of TcpP proteolysis promoting virulence gene expression in\n                    <i>Vibrio cholerae<\/i>"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0491-0549","authenticated-orcid":false,"given":"Lucas M.","family":"Demey","sequence":"first","affiliation":[{"name":"Department of Microbiology & Molecular Genetics, Michigan State University","place":["East Lansing, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritam","family":"Sinha","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Molecular Genetics, Michigan State University","place":["East Lansing, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3617-7011","authenticated-orcid":false,"given":"Victor J.","family":"DiRita","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Molecular Genetics, Michigan State University","place":["East Lansing, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.3733-3739.1999"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004405"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.9.2833"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2204"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.7.2634"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001102"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.21.6974-6980.1992"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01263.x"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02111.x"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.12.5403"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90430-2"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03398.x"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.2.730"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.5371901.x"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(03)00222-3"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00566-10"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03229.x"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00193-10"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014640108"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.12.038"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052694299"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.1.178-188.2001"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.1.265"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02518.x"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.24.8309-8316.2004"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13069"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505818102"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(00)80675-3"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3611718.x"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1008902"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100464200"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040002"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04870.x"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01946.x"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1369-5274(00)00140-5"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0611567104"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.06.039"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(04)00454-4"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307709101"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.17.5384-5388.1999"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.10.3177-3184.1992"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00266-23"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2203176119"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.15718"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187846"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14510"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14125"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13699"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66050-5"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14673"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00036-14"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13914"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2019.183050"},{"key":"e_1_3_5_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2017.05.027"},{"key":"e_1_3_5_56_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1945010"},{"key":"e_1_3_5_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.16"},{"key":"e_1_3_5_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2002214"},{"key":"e_1_3_5_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.1.313-323.2006"},{"key":"e_1_3_5_60_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.32.110601.141803"},{"key":"e_1_3_5_61_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.E600002-JLR200"},{"key":"e_1_3_5_62_2","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400010308"},{"key":"e_1_3_5_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/42408"},{"key":"e_1_3_5_64_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar00021a004"},{"key":"e_1_3_5_65_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1515607112"},{"key":"e_1_3_5_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.10.3129-3135.1999"},{"key":"e_1_3_5_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90206-e"},{"key":"e_1_3_5_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)69262-8"},{"key":"e_1_3_5_69_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1971.tb01334.x"},{"key":"e_1_3_5_70_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.7.3377"},{"key":"e_1_3_5_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42447-1"},{"key":"e_1_3_5_72_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07476.x"},{"key":"e_1_3_5_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01415-17"},{"key":"e_1_3_5_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00289-1"},{"key":"e_1_3_5_75_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218039110"},{"key":"e_1_3_5_76_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02409-14"},{"key":"e_1_3_5_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01029-15"},{"key":"e_1_3_5_78_2","doi-asserted-by":"publisher","DOI":"10.1128\/am.16.4.549-552.1968"},{"key":"e_1_3_5_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.52.4.794-801.1986"},{"key":"e_1_3_5_80_2","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12785"},{"key":"e_1_3_5_81_2","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.154"},{"key":"e_1_3_5_82_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00617a031"},{"key":"e_1_3_5_83_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.3.5.549"},{"key":"e_1_3_5_84_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi901890a"},{"key":"e_1_3_5_85_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2017.06.015"},{"key":"e_1_3_5_86_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.9.6236"},{"key":"e_1_3_5_87_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2007.11.003"},{"key":"e_1_3_5_88_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01328-3"},{"key":"e_1_3_5_89_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0631579100"},{"key":"e_1_3_5_90_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00338-15"},{"key":"e_1_3_5_91_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2005.06.004"},{"key":"e_1_3_5_92_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02213-21"},{"key":"e_1_3_5_93_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.6459"},{"key":"e_1_3_5_94_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12173"},{"key":"e_1_3_5_95_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008678"},{"key":"e_1_3_5_96_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01073-09"},{"key":"e_1_3_5_97_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00832-12"},{"key":"e_1_3_5_98_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00419-15"},{"key":"e_1_3_5_99_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1491-1497.2000"},{"key":"e_1_3_5_100_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.3743-3748.2004"},{"key":"e_1_3_5_101_2","doi-asserted-by":"publisher","DOI":"10.1159\/000090346"},{"key":"e_1_3_5_102_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13376"},{"key":"e_1_3_5_103_2","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.0800.121"},{"key":"e_1_3_5_104_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01028-13"},{"key":"e_1_3_5_105_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00889-12"},{"key":"e_1_3_5_106_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01123-18"},{"key":"e_1_3_5_107_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42252-1"},{"key":"e_1_3_5_108_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02294-12"},{"key":"e_1_3_5_109_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010632"},{"key":"e_1_3_5_110_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13170"},{"key":"e_1_3_5_111_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.3.1288-1293.1989"},{"key":"e_1_3_5_112_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005145"},{"key":"e_1_3_5_113_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2009.08.019"},{"key":"e_1_3_5_114_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2006.05.013"},{"key":"e_1_3_5_115_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-3480-1"},{"key":"e_1_3_5_116_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(05)73006-X"},{"key":"e_1_3_5_117_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2007-0157"},{"key":"e_1_3_5_118_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(07)71585-0"},{"key":"e_1_3_5_119_2","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:2005047"},{"key":"e_1_3_5_120_2","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2016.1222996"},{"key":"e_1_3_5_121_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu7085289"},{"key":"e_1_3_5_122_2","doi-asserted-by":"publisher","DOI":"10.1017\/jns.2015.13"},{"key":"e_1_3_5_123_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0b013e3281e2c9d4"},{"key":"e_1_3_5_124_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/79.6.935"},{"key":"e_1_3_5_125_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-008-3269-z"},{"key":"e_1_3_5_126_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-001-0746-0"},{"key":"e_1_3_5_127_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2004.08.002"},{"key":"e_1_3_5_128_2","doi-asserted-by":"publisher","DOI":"10.1079\/pns2005418"},{"key":"e_1_3_5_129_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2006.02.009"},{"key":"e_1_3_5_130_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2021.108597"},{"key":"e_1_3_5_131_2","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201800448"},{"key":"e_1_3_5_132_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104443"},{"key":"e_1_3_5_133_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1453-3"},{"key":"e_1_3_5_134_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01435-06"},{"key":"e_1_3_5_135_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07244.x"},{"key":"e_1_3_5_136_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01824-14"},{"key":"e_1_3_5_137_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00409-09"},{"key":"e_1_3_5_138_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02068-14"},{"key":"e_1_3_5_139_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00722-13"},{"key":"e_1_3_5_140_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00663-10"},{"key":"e_1_3_5_141_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.11.2103"},{"key":"e_1_3_5_142_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2020.03.008"},{"key":"e_1_3_5_143_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c02899"},{"key":"e_1_3_5_144_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-019-01659-y"},{"key":"e_1_3_5_145_2","doi-asserted-by":"publisher","unstructured":"LB solid or liquid medium: Cold spring harbor protocols. 2016. doi:10.1101\/pdb.rec088203","DOI":"10.1101\/pdb.rec088203"},{"key":"e_1_3_5_146_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00793-8"},{"key":"e_1_3_5_147_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220915"},{"key":"e_1_3_5_148_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00403-13"},{"key":"e_1_3_5_149_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.15.3.704-712.1977"},{"key":"e_1_3_5_150_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_3_5_151_2","first-page":"9","article-title":"Determination of ctxAB expression in Vibrio cholerae classical and El Tor strains using real-time PCR","volume":"2","author":"Marashi SMA","year":"2013","unstructured":"Marashi SMA, et al.. 2013. Determination of ctxAB expression in Vibrio cholerae classical and El Tor strains using real-time PCR. Int J Mol Cell Med 2:9\u201313.","journal-title":"Int J Mol Cell Med"},{"key":"e_1_3_5_152_2","volume-title":"Experiments in molecular genetics","author":"Miller JH","year":"1974","unstructured":"Miller JH. 1974. Experiments in molecular genetics. Cold spring harbor laboratory."},{"key":"e_1_3_5_153_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-245-2_22"},{"key":"e_1_3_5_154_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.10.029"},{"key":"e_1_3_5_155_2","doi-asserted-by":"publisher","DOI":"10.3791\/2518"},{"key":"e_1_3_5_156_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62614"},{"key":"e_1_3_5_157_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.157.1.233-239.1984"},{"key":"e_1_3_5_158_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2018.11.015"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00721-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00721-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T13:37:47Z","timestamp":1791553067000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mbio.00721-24"}},"subtitle":[],"editor":[{"given":"K. Heran","family":"Darwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,8,14]]},"references-count":157,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8,14]]}},"alternative-id":["10.1128\/mbio.00721-24"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00721-24","relation":{},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,14]]},"assertion":[{"value":"2024-04-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00721-24"}}