{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T15:48:50Z","timestamp":1793634530552,"version":"4.5.1"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T00:00:00Z","timestamp":1530748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2018,7,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Flagellum-driven motility of\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Typhimurium facilitates host colonization. However, the large extracellular flagellum is also a prime target for the immune system. As consequence, expression of flagella is bistable within a population of\n            <jats:italic>Salmonella<\/jats:italic>\n            , resulting in flagellated and nonflagellated subpopulations. This allows the bacteria to maximize fitness in hostile environments. The degenerate EAL domain protein RflP (formerly YdiV) is responsible for the bistable expression of flagella by directing the flagellar master regulatory complex FlhD\n            <jats:sub>4<\/jats:sub>\n            C\n            <jats:sub>2<\/jats:sub>\n            with respect to proteolytic degradation. Information concerning the environmental cues controlling expression of\n            <jats:italic>rflP<\/jats:italic>\n            and thus about the bistable flagellar biosynthesis remains ambiguous. Here, we demonstrated that RflP responds to cell envelope stress and alterations of outer membrane integrity. Lipopolysaccharide (LPS) truncation mutants of\n            <jats:italic>Salmonella<\/jats:italic>\n            Typhimurium exhibited increasing motility defects due to downregulation of flagellar gene expression. Transposon mutagenesis and genetic profiling revealed that \u03c3\n            <jats:sup>24<\/jats:sup>\n            (RpoE) and Rcs phosphorelay-dependent cell envelope stress response systems sense modifications of the lipopolysaccaride, low pH, and activity of the complement system. This subsequently results in activation of RflP expression and degradation of FlhD\n            <jats:sub>4<\/jats:sub>\n            C\n            <jats:sub>2<\/jats:sub>\n            via ClpXP. We speculate that the presence of diverse hostile environments inside the host might result in cell envelope damage and would thus trigger the repression of resource-costly and immunogenic flagellum biosynthesis via activation of the cell envelope stress response.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Pathogenic bacteria such as\n            <jats:italic>Salmonella<\/jats:italic>\n            Typhimurium sense and adapt to a multitude of changing and stressful environments during host infection. At the initial stage of gastrointestinal colonization,\n            <jats:italic>Salmonella<\/jats:italic>\n            uses flagellum-mediated motility to reach preferred sites of infection. However, the flagellum also constitutes a prime target for the host\u2019s immune response. Accordingly, the pathogen needs to determine the spatiotemporal stage of infection and control flagellar biosynthesis in a robust manner. We found that\n            <jats:italic>Salmonella<\/jats:italic>\n            uses signals from cell envelope stress-sensing systems to turn off production of flagella. We speculate that downregulation of flagellum synthesis after cell envelope damage in hostile environments aids survival of\n            <jats:italic>Salmonella<\/jats:italic>\n            during late stages of infection and provides a means to escape recognition by the immune system.\n          <\/jats:p>","DOI":"10.1128\/mbio.00736-17","type":"journal-article","created":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T10:04:05Z","timestamp":1525169045000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":87,"title":["Regulation of Flagellum Biosynthesis in Response to Cell Envelope Stress in\n            <i>Salmonella enterica<\/i>\n            Serovar Typhimurium"],"prefix":"10.1128","volume":"9","author":[{"given":"Imke","family":"Sp\u00f6ring","sequence":"first","affiliation":[{"name":"Junior Research Group Infection Biology of Salmonella, Helmholtz Centre for Infection Research, Braunschweig, 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