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Among the many approaches investigated to limit the diseases caused by these viruses are attempts to make mosquitos resistant to virus infection. Coinfection of mosquitos with the bacterium\n                    <jats:named-content content-type=\"genus-species\">Wolbachia pipientis<\/jats:named-content>\n                    from supergroup A is a recent strategy employed to reduce the capacity for major vectors in the\n                    <jats:italic>Aedes<\/jats:italic>\n                    mosquito genus to transmit viruses, including dengue virus (DENV), Chikungunya virus (CHIKV), and Zika virus (ZIKV). Recently, a supergroup B\n                    <jats:italic>Wolbachia w<\/jats:italic>\n                    Stri, isolated from\n                    <jats:named-content content-type=\"genus-species\">Laodelphax striatellus<\/jats:named-content>\n                    , was shown to inhibit multiple lineages of ZIKV in\n                    <jats:named-content content-type=\"genus-species\">Aedes\u00a0albopictus<\/jats:named-content>\n                    cells. Here, we show that\n                    <jats:italic>w<\/jats:italic>\n                    Stri blocks the growth of positive-sense RNA viruses DENV, CHIKV, ZIKV, and yellow fever virus by greater than 99.9%.\n                    <jats:italic>w<\/jats:italic>\n                    Stri presence did not affect the growth of the negative-sense RNA viruses LaCrosse virus or vesicular stomatitis virus. Investigation of the stages of the ZIKV life cycle inhibited by\n                    <jats:italic>w<\/jats:italic>\n                    Stri identified two distinct blocks in viral replication. We found a reduction of ZIKV entry into\n                    <jats:italic>w<\/jats:italic>\n                    Stri-infected cells. This was partially rescued by the addition of a cholesterol-lipid supplement. Independent of entry, transfected viral genome was unable to replicate in\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    -infected cells. RNA transfection and metabolic labeling studies suggested that this replication defect is at the level of RNA translation, where we saw a 66% reduction in mosquito protein synthesis in\n                    <jats:italic>w<\/jats:italic>\n                    Stri-infected cells. This study\u2019s findings increase the potential for application of\n                    <jats:italic>w<\/jats:italic>\n                    Stri to block additional arboviruses and also identify specific blocks in viral infection caused by\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    coinfection.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    Dengue, Zika, and yellow fever viruses are mosquito-transmitted diseases that have spread throughout the world, causing millions of infections and thousands of deaths each year. Existing programs that seek to contain these diseases through elimination of the mosquito population have so far failed, making it crucial to explore new ways of limiting the spread of these viruses. Here, we show that introduction of an insect symbiont,\n                    <jats:italic>Wolbachia w<\/jats:italic>\n                    Stri, into mosquito cells is highly effective at reducing yellow fever virus, dengue virus, Zika virus, and Chikungunya virus production. Reduction of virus replication was attributable to decreases in entry and a strong block of virus gene expression at the translational level. These findings expand the potential use of\n                    <jats:italic>Wolbachia w<\/jats:italic>\n                    Stri to block viruses and identify two separate steps for limiting virus replication in mosquitos that could be targeted via microbes or other means as an antiviral strategy.\n                  <\/jats:p>","DOI":"10.1128\/mbio.00738-18","type":"journal-article","created":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T05:35:54Z","timestamp":1526880954000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["<i>Wolbachia w<\/i>\n                    Stri Blocks Zika Virus Growth at Two Independent Stages of Viral Replication"],"prefix":"10.1128","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5054-8029","authenticated-orcid":false,"given":"M. J.","family":"Schultz","sequence":"first","affiliation":[{"name":"Department of Biology, Boston University, Boston, Massachusetts, USA"},{"name":"Molecular Biology, Cell Biology &amp; Biochemistry Graduate Program, Boston University, Boston, Massachusetts, USA"},{"name":"National Emerging Infectious Diseases Laboratories, Boston University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. L.","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. N.","family":"Gray","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Isern","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. F.","family":"Michael","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0191-7948","authenticated-orcid":false,"given":"H. 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