{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:45:35Z","timestamp":1790682335896,"version":"4.1.0"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T00:00:00Z","timestamp":1560211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T00:00:00Z","timestamp":1561420800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["LTFCOFUND2013"],"award-info":[{"award-number":["LTFCOFUND2013"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["GA-2013-609409"],"award-info":[{"award-number":["GA-2013-609409"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["H2020-MSCA-IF-2015 703379"],"award-info":[{"award-number":["H2020-MSCA-IF-2015 703379"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","award":["ALTF 1497-2015"],"award-info":[{"award-number":["ALTF 1497-2015"]}],"id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012623","name":"Federation of European Biochemical Societies","doi-asserted-by":"publisher","award":["LTF"],"award-info":[{"award-number":["LTF"]}],"id":[{"id":"10.13039\/100012623","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,6,25]]},"abstract":"<jats:p>\n                    <jats:named-content content-type=\"genus-species\">Anopheles gambiae<\/jats:named-content>\n                    mosquitos are the main vectors of malaria, threatening around half of the world\u2019s population. The bacterial symbiont\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    can interfere with disease transmission by other important insect vectors, but until recently, it was thought to be absent from natural\n                    <jats:italic>A. gambiae<\/jats:italic>\n                    populations. Here, we critically analyze the genomic, metagenomic, PCR, imaging, and phenotypic data presented in support of the presence of natural\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    infections in\n                    <jats:named-content content-type=\"genus-species\">A. gambiae<\/jats:named-content>\n                    . We find that they are insufficient to diagnose\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    infections and argue for the need of obtaining robust data confirming basic\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    characteristics in this system. Determining the\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    infection status of\n                    <jats:italic>Anopheles<\/jats:italic>\n                    is critical due to its potential to influence\n                    <jats:italic>Anopheles<\/jats:italic>\n                    population structure and\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    transmission.\n                  <\/jats:p>","DOI":"10.1128\/mbio.00784-19","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T10:20:12Z","timestamp":1560162012000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Is Anopheles gambiae a Natural Host of\n                    <i>Wolbachia<\/i>\n                    ?"],"prefix":"10.1128","volume":"10","author":[{"given":"Ewa","family":"Chrostek","sequence":"first","affiliation":[{"name":"Max Planck Institute for Infection Biology, Berlin, Germany"},{"name":"Institute of Integrative Biology, University of Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Gerth","sequence":"additional","affiliation":[{"name":"Institute of Integrative Biology, University of Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2015.0249"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1969"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(73)90141-9"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2001.1876"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/216383a0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911476107"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1209609"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1162418"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.11.042"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-8727-1_24"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10355"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002688"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003115"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.12688\/gatesopenres.12844.1"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001143"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-39.4.562"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585(2000)037[0340:DADOWI]2.0.CO;2"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-41.2.255"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01578-06"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00207-09"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002043"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1236192"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408888111"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4985"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep22806"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11772"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1716181114"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","unstructured":"Ayala D. 2018. Natural Wolbachia infections are common in the major malaria vectors in Central Africa. bioRxiv http:\/\/biorxiv.org\/content\/early\/2018\/06\/11\/343715.abstract.","DOI":"10.1101\/343715"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14765.2"},{"key":"e_1_3_2_33_2","unstructured":"Ag100G Consortium. 2015. Ag1000G phase 1 AR3 data release. MalariaGEN http:\/\/www.malariagen.net\/data\/ag1000g-phase1-AR3."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.5486"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2000.1139"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003129"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep17952"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/insects8010001"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-14-121"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2009.04321.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00236-18"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.7144908"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.222228199"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-33"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1142490"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02237"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiy058"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2017.09.005"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2559-z"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13877"},{"key":"e_1_3_2_51_2","doi-asserted-by":"crossref","unstructured":"Carvajal T Hashimoto K Harnandika RK Amalin D Watanabe K. 2018. Detection of Wolbachia in field-collected mosquito vector Aedes aegypti. bioRxiv http:\/\/biorxiv.org\/content\/early\/2018\/09\/08\/408856.abstract.","DOI":"10.1101\/408856"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjy084"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02160"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.3529"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-014-0087-z"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110808"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0202-y"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003896"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01727-12"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004369"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00784-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.00784-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:45:17Z","timestamp":1663634717000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.00784-19"}},"subtitle":[],"editor":[{"given":"Nancy A.","family":"Moran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,6,25]]},"references-count":59,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,6,25]]}},"alternative-id":["10.1128\/mBio.00784-19"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00784-19","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.735978101.793564466","asserted-by":"object"}],"has-preprint":[{"id-type":"doi","id":"10.1101\/491449","asserted-by":"object"}]},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,25]]},"assertion":[{"value":"2019-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00784-19"}}