{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T03:13:41Z","timestamp":1793330021107,"version":"4.5.0"},"reference-count":73,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | National Institutes of Health","award":["AI127338"],"award-info":[{"award-number":["AI127338"]}]},{"name":"HHS | National Institutes of Health","award":["AI048071"],"award-info":[{"award-number":["AI048071"]}]},{"DOI":"10.13039\/100012403","name":"Texas Biomedical Research Institute","doi-asserted-by":"crossref","award":["20-04866"],"award-info":[{"award-number":["20-04866"]}],"id":[{"id":"10.13039\/100012403","id-type":"DOI","asserted-by":"crossref"}]},{"name":"HHS | NIH | National Center for Research Resources","award":["C06 RR013556"],"award-info":[{"award-number":["C06 RR013556"]}]},{"name":"Wellcome Trust of Great Britain","award":["099992\/Z\/12\/Z"],"award-info":[{"award-number":["099992\/Z\/12\/Z"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2024,7,17]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Piperaquine (PPQ) is widely used in combination with dihydroartemisinin as a first-line treatment against malaria. Multiple genetic drivers of PPQ resistance have been reported, including mutations in the\n              <jats:italic>Plasmodium falciparum chloroquine resistance transporter<\/jats:italic>\n              (\n              <jats:italic>pfcrt<\/jats:italic>\n              ) and increased copies of\n              <jats:italic>plasmepsin II\/III<\/jats:italic>\n              (\n              <jats:italic>pm2\/3<\/jats:italic>\n              ). We generated a cross between a Cambodia-derived multidrug-resistant KEL1\/PLA1 lineage isolate (KH004) and a drug-susceptible Malawian parasite (Mal31). Mal31 harbors a wild-type (3D7-like)\n              <jats:italic>pfcrt<\/jats:italic>\n              allele and a single copy of\n              <jats:italic>pm2\/3<\/jats:italic>\n              , while KH004 has a chloroquine-resistant (Dd2-like)\n              <jats:italic>pfcrt<\/jats:italic>\n              allele with an additional G367C substitution and multiple copies of\n              <jats:italic>pm2\/3<\/jats:italic>\n              . We recovered 104 unique recombinant parasites and examined a targeted set of progeny representing all possible combinations of variants at\n              <jats:italic>pfcrt<\/jats:italic>\n              and\n              <jats:italic>pm2\/3<\/jats:italic>\n              . We performed a detailed analysis of competitive fitness and a range of PPQ susceptibility phenotypes with these progenies, including PPQ survival assay, area under the dose response curve, and a limited point IC\n              <jats:sub>50<\/jats:sub>\n              . We find that inheritance of the KH004\n              <jats:italic>pfcrt<\/jats:italic>\n              allele is required for reduced PPQ sensitivity, whereas copy number variation in\n              <jats:italic>pm2\/3<\/jats:italic>\n              further decreases susceptibility but does not confer resistance in the absence of additional mutations in\n              <jats:italic>pfcrt<\/jats:italic>\n              . A deep investigation of genotype-phenotype relationships demonstrates that progeny clones from experimental crosses can be used to understand the relative contributions\n              <jats:italic>of pfcrt<\/jats:italic>\n              ,\n              <jats:italic>pm2\/3<\/jats:italic>\n              , and parasite genetic background to a range of PPQ-related traits. Additionally, we find that the resistance phenotype associated with parasites inheriting the G367C substitution in pfcrt is consistent with previously validated PPQ resistance mutations in this transporter.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                Resistance to piperaquine, used in combination with dihydroartemisinin, has emerged in Cambodia and threatens to spread to other malaria-endemic regions. Understanding the causal mutations of drug resistance and their impact on parasite fitness is critical for surveillance and intervention and can also reveal new avenues to limiting the evolution and spread of drug resistance. An experimental genetic cross is a powerful tool for pinpointing the genetic determinants of key drug resistance and fitness phenotypes and has the distinct advantage of quantifying the effects of naturally evolved genetic variation. Our study was strengthened since the full range of copies of KH004\n                <jats:italic>pm2\/3<\/jats:italic>\n                was inherited among the progeny clones, allowing us to directly test the role of the\n                <jats:italic>pm2\/3<\/jats:italic>\n                copy number on resistance-related phenotypes in the context of a unique\n                <jats:italic>pfcrt<\/jats:italic>\n                allele. Our multigene model suggests an important role for both loci in the evolution of this multidrug-resistant parasite lineage.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/mbio.00805-24","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T13:08:54Z","timestamp":1719234534000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["A\n            <i>Plasmodium falciparum<\/i>\n            genetic cross reveals the contributions of\n            <i>pfcrt<\/i>\n            and\n            <i>plasmepsin II\/III<\/i>\n            to piperaquine drug resistance"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4917-7205","authenticated-orcid":false,"given":"John","family":"Kane","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Li","sequence":"additional","affiliation":[{"name":"Disease Intervention and Prevention Program, Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2847-393X","authenticated-orcid":false,"given":"Sudhir","family":"Kumar","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina A.","family":"Button-Simons","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katelyn M.","family":"Vendrely Brenneman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haley","family":"Dahlhoff","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mackenzie A. C.","family":"Sievert","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A.","family":"Checkley","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas A.","family":"Shoue","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puspendra P.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biley A.","family":"Abatiyow","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meseret T.","family":"Haile","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shalini","family":"Nair","sequence":"additional","affiliation":[{"name":"Disease Intervention and Prevention Program, Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Reyes","sequence":"additional","affiliation":[{"name":"Disease Intervention and Prevention Program, Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupam","family":"Tripura","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine Research Building, University of Oxford Old Road Campus, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Peto","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine Research Building, University of Oxford Old Road Campus, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dysoley","family":"Lek","sequence":"additional","affiliation":[{"name":"National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia"},{"name":"School of Public Health, National Institute of Public Health, Phnom Penh, Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angana","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"},{"name":"Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan H. I.","family":"Kappe","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"},{"name":"Department of Pediatrics, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehul","family":"Dhorda","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine Research Building, University of Oxford Old Road Campus, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Standwell C.","family":"Nkhoma","sequence":"additional","affiliation":[{"name":"BEI Resources, American Type Culture Collection (ATCC), Manassas, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian H.","family":"Cheeseman","sequence":"additional","affiliation":[{"name":"Host Pathogen Interactions Program, Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley M.","family":"Vaughan","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"},{"name":"Department of Pediatrics, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0191-0204","authenticated-orcid":false,"given":"Timothy J. C.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Disease Intervention and Prevention Program, Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3516-9829","authenticated-orcid":false,"given":"Michael T.","family":"Ferdig","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60024-0"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4381"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(87)90147-5"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2009.04.004"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30048-8"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30228-0"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2012.08.002"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30068-9"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30392-5"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.08714"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.18681.1"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04104-z"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw228"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12876"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30409-1"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33873-x"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-015-0539-5"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)00487-9"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30415-7"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02309-17"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2018.11.004"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evx126"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-03339-w"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05652-0"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30414-5"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adi2364"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010926"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiac365"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010278"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02731-18"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30391-3"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30543-2"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1795-x"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1507142112"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3189"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00813"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-019-2934-4"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008453"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-023-01377-z"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa628"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3432"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.203711.115"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104095"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.878496"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02210-1"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01832-23"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011436"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(23)00502-9"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.2c00669"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00303-17"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2006.03.008"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00605-18"},{"key":"e_1_3_5_55_2","doi-asserted-by":"crossref","unstructured":"Neumann C Kulik L. 2014. Package\"EloRating\" (0.43)","DOI":"10.15358\/0340-1650_2014_4_209"},{"key":"e_1_3_5_56_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi8010658"},{"key":"e_1_3_5_57_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.19-0379"},{"key":"e_1_3_5_58_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.21-1268"},{"key":"e_1_3_5_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00697-1"},{"key":"e_1_3_5_60_2","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20210001"},{"key":"e_1_3_5_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep33891"},{"key":"e_1_3_5_62_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2532-x"},{"key":"e_1_3_5_63_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.168286.113"},{"key":"e_1_3_5_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11174"},{"key":"e_1_3_5_65_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2349-7"},{"key":"e_1_3_5_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2020.07.009"},{"key":"e_1_3_5_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_5_68_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1061"},{"key":"e_1_3_5_69_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi0313s48"},{"key":"e_1_3_5_70_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-3139-6"},{"key":"e_1_3_5_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anbehav.2011.07.016"},{"key":"e_1_3_5_72_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-03249-x"},{"key":"e_1_3_5_73_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/159.1.371"},{"key":"e_1_3_5_74_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg112"},{"key":"e_1_3_5_75_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/138.3.963"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00805-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00805-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T13:05:44Z","timestamp":1721221544000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mbio.00805-24"}},"subtitle":[],"editor":[{"given":"L. David","family":"Sibley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,7,17]]},"references-count":73,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7,17]]}},"alternative-id":["10.1128\/mbio.00805-24"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00805-24","relation":{},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,17]]},"assertion":[{"value":"2024-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00805-24"}}