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The T4ASSs, represented by the\n                    <jats:named-content content-type=\"genus-species\">Agrobacterium tumefaciens<\/jats:named-content>\n                    VirB\/VirD4\n                    <jats:sub>T4SS<\/jats:sub>\n                    , include \u201cminimized\u201d machines assembled from 12 VirB- and VirD4-like subunits and compositionally larger systems such as the\n                    <jats:named-content content-type=\"genus-species\">Helicobacter pylori<\/jats:named-content>\n                    Cag\n                    <jats:sub>T4SS<\/jats:sub>\n                    . T4BSSs encompass systems closely related in subunit composition to the\n                    <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n                    Dot\/Icm\n                    <jats:sub>T4SS<\/jats:sub>\n                    . Here, we present structures of native and mutant\n                    <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n                    Cag machines determined by\n                    <jats:italic>in situ<\/jats:italic>\n                    cryoelectron tomography. We identify distinct outer and inner membrane complexes and, for the first time, visualize structural contributions of all three \u201csignature\u201d ATPases of T4SSs at the cytoplasmic entrance of the translocation channel. Despite their evolutionary divergence, the Cag\n                    <jats:sub>T4SS<\/jats:sub>\n                    aligns structurally much more closely to the Dot\/Icm\n                    <jats:sub>T4SS<\/jats:sub>\n                    than an available VirB\/VirD4 subcomplex. Our findings highlight the diversity of T4SSs and suggest a structural classification scheme in which T4SSs are grouped as minimized VirB\/VirD4-like or larger Cag-like and Dot\/Icm-like systems.\n                  <\/jats:p>","DOI":"10.1128\/mbio.00849-19","type":"journal-article","created":{"date-parts":[[2019,5,13]],"date-time":"2019-05-13T10:01:24Z","timestamp":1557741684000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["<i>In Situ<\/i>\n                    Molecular Architecture of the Helicobacter pylori Cag Type IV Secretion System"],"prefix":"10.1128","volume":"10","author":[{"given":"Bo","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, McGovern Medical School, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratick","family":"Khara","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, McGovern Medical School, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqiang","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, McGovern Medical School, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aung Soe","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arwen E.","family":"Frick-Cheng","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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