{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:02:49Z","timestamp":1790812969903,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI044198"],"award-info":[{"award-number":["AI044198"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI151212"],"award-info":[{"award-number":["AI151212"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI151212"],"award-info":[{"award-number":["AI151212"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI141346"],"award-info":[{"award-number":["AI141346"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2022,8,30]]},"abstract":"<jats:p>\n            Small RNAs (sRNAs) are a class of regulatory RNAs that play important roles in bacterial physiology and pathogenesis. In the intracellular bacterium\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            , however, sRNAs are poorly understood, and functional studies have been limited to a heterologous system.\n          <\/jats:p>","DOI":"10.1128\/mbio.00864-22","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T12:30:37Z","timestamp":1655814637000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["A Reverse Genetic Approach for Studying sRNAs in Chlamydia trachomatis"],"prefix":"10.1128","volume":"13","author":[{"given":"Kevin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Sheehan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuper","family":"Ramirez","sequence":"additional","affiliation":[{"name":"Department of Developmental and Cell Biology, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asha","family":"Densi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed","family":"Rizvi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roseleen","family":"Ekka","sequence":"additional","affiliation":[{"name":"Department of Developmental and Cell Biology, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1354-507X","authenticated-orcid":true,"given":"Christine","family":"S\u00fctterlin","sequence":"additional","affiliation":[{"name":"Department of Developmental and Cell Biology, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5361-583X","authenticated-orcid":true,"given":"Ming","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, California, USA"},{"name":"Department of Medicine, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"CDC. 2021. STI prevalence incidence and cost estimates infographic. Centers for Disease Control and Prevention https:\/\/www.cdc.gov\/std\/statistics\/prevalence-2020-at-a-glance.htm."},{"key":"e_1_3_2_3_2","unstructured":"Annual statistics from the National Notifiable Diseases Surveillance System (NNDSS). https:\/\/wonder.cdc.gov\/nndss\/nndss_annual_tables_menu.asp."},{"key":"e_1_3_2_4_2","first-page":"2301","volume-title":"Mandell, Douglas, and Bennett\u2019s principles and practice of infectious diseases","author":"Batteiger BE","year":"2019","unstructured":"Batteiger BE, Tan M. 2019. Chlamydia trachomatis (trachoma and urogenital infections), p 2301\u20132319. In Bennett JE, Dolin R, Blaser MJ (ed), Mandell, Douglas, and Bennett\u2019s principles and practice of infectious diseases, 9th ed Elsevier Inc., Philadelphia, PA.","edition":"9"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.1.143-190.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02432-0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1331135100"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.21775\/9781912530281.10"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.14.4274-4281.1993"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.9.3937"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a003798"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.08.022"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.043"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00032-10"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1065"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp1032"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04949.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08100.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01290-13"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1748-7188-6-26"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","unstructured":"Mercier N Pr\u00e9vost K Mass\u00e9 E Romby P Caldelari I Lalaouna D. 2021. MS2-affinity purification coupled with RNA sequencing in Gram-positive bacteria. JoVE doi:10.3791\/61731.","DOI":"10.3791\/61731"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2018.08.022"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gdata.2015.05.033"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx279"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku359"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn544"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky329"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl1040"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku317"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2018.01.002"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpmc.31"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R112.441386"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00059"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-019-6059-5"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00108-21"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1156"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090817-062607"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.11.011"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2009.07.006"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1201398109"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01075-15"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2020.194504"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz149"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116593"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl697"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2018.10.003"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1186\/1754-1611-8-5"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002258"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401044"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00729-20"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00864-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.00864-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T13:23:57Z","timestamp":1661865837000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mbio.00864-22"}},"subtitle":[],"editor":[{"given":"Gisela","family":"Storz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,8,30]]},"references-count":49,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,8,30]]}},"alternative-id":["10.1128\/mbio.00864-22"],"URL":"https:\/\/doi.org\/10.1128\/mbio.00864-22","relation":{},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,30]]},"assertion":[{"value":"2022-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00864-22"}}