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TonB-dependent transporters are a conserved set of proteins that serve crucial functions for bacterial survival within the host. In this study, binding between the gonococcal transporter, TdfH, and calprotectin was determined to be of high affinity and host restricted. The current study identified a preferential TdfH interaction at the calprotectin dimer interface. An antigonococcal therapeutic could potentially block this site on calprotectin, interrupting Zn uptake by\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            and thereby prohibiting continued bacterial growth. We describe protein-protein interactions between TdfH and calprotectin, and our findings provide the building blocks for future therapeutic or prophylactic targets.\n          <\/jats:p>","DOI":"10.1128\/mbio.00949-20","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T13:15:20Z","timestamp":1590412520000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Molecular Insight into TdfH-Mediated Zinc Piracy from Human Calprotectin by Neisseria gonorrhoeae"],"prefix":"10.1128","volume":"11","author":[{"given":"Michael T.","family":"Kammerman","sequence":"first","affiliation":[{"name":"Center for Translational Immunology, Institute for Biomedical Sciences, Georgia State University, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aloke","family":"Bera","sequence":"additional","affiliation":[{"name":"Markey Center for Structural Biology, Department of Biological Science, Purdue University, West Lafayette, Indiana, USA"},{"name":"Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, Indiana, USA"},{"name":"Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Runrun","family":"Wu","sequence":"additional","affiliation":[{"name":"Markey Center for Structural Biology, Department of Biological Science, Purdue University, West Lafayette, Indiana, USA"},{"name":"Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, Indiana, USA"},{"name":"Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone A.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA"},{"name":"Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA"},{"name":"Center for Structural Biology, Vanderbilt University, Nashville, Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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