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To accomplish this, gammaherpesviruses employ an array of gene products, including miRNAs, short noncoding RNAs that bind to and repress protein synthesis from specific target mRNAs. The\n            <jats:italic>in vivo<\/jats:italic>\n            relevance of repression of targets of gammaherpesvirus miRNAs remains highly elusive. Here, we identified a murine gammaherpesvirus miRNA as critical for\n            <jats:italic>in vivo<\/jats:italic>\n            infection and validated the host mRNA\n            <jats:italic>EWSR1<\/jats:italic>\n            (Ewing sarcoma breakpoint region 1) as the predominant target for this miRNA. Using a novel technology, we demonstrated that repression of\n            <jats:italic>EWSR1<\/jats:italic>\n            was essential for\n            <jats:italic>in vivo<\/jats:italic>\n            infection of the critical B cell reservoir. These findings provide the first\n            <jats:italic>in vivo<\/jats:italic>\n            demonstration of the significance of repression of a specific host mRNA by a gammaherpesvirus miRNA.\n          <\/jats:p>","DOI":"10.1128\/mbio.00996-19","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T14:37:43Z","timestamp":1564411063000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["A Gammaherpesvirus MicroRNA Targets EWSR1 (Ewing Sarcoma Breakpoint Region 1)\n            <i>In Vivo<\/i>\n            To Promote Latent Infection of Germinal Center B Cells"],"prefix":"10.1128","volume":"10","author":[{"given":"Yiping","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, UF Health Cancer Center, College of Medicine, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily R.","family":"Feldman","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, UF Health Cancer Center, College of Medicine, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Whitney L.","family":"Bullard","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, UF Health Cancer Center, College of Medicine, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Tibbetts","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, UF Health Cancer Center, College of Medicine, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens5010018"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2888"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra032015"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5414.641"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01656-12"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.4.2974-2982.1999"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/35095584"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.006"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060308-103103"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.40"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003018"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096781"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth746"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2013.05.013"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408192102"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020023"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2326106"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.14.9301-9305.2005"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02138-17"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.043"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.03.043"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01667-16"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.04.003"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/v4091687"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-072710-081639"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-43.1.32"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-73-12-3275"},{"key":"e_1_3_2_29_2","first-page":"818","article-title":"Lymphoproliferative disease in mice infected with murine gammaherpesvirus 68","volume":"145","author":"Sunil-Chandra NP","year":"1994","unstructured":"Sunil-Chandra NP, Arno J, Fazakerley J, Nash AA. 1994. 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