{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:09:54Z","timestamp":1791846594201,"version":"4.3.4"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T00:00:00Z","timestamp":1590451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T00:00:00Z","timestamp":1593475200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI145069"],"award-info":[{"award-number":["R01AI145069"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R37AI21150"],"award-info":[{"award-number":["R37AI21150"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2020,6,30]]},"abstract":"<jats:p>\n                    <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n                    has become a highly antimicrobial-resistant Gram-negative pathogen. Multidrug efflux is a major mechanism that\n                    <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n                    uses to counteract the action of multiple classes of antibiotics. It appears that gonococci bearing mosaic-like sequences within the gene\n                    <jats:italic>mtrD<\/jats:italic>\n                    , encoding the most predominant and clinically important transporter of any gonococcal multidrug efflux pump, significantly elevate drug resistance and enhance transport function. Here, we report cryo-electron microscopy (EM) structures of\n                    <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n                    MtrD carrying a mosaic-like sequence that allow us to understand the mechanism of drug recognition. Our work will ultimately inform structure-guided drug design for inhibiting these critical multidrug efflux pumps.\n                  <\/jats:p>","DOI":"10.1128\/mbio.00996-20","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T09:15:20Z","timestamp":1590398120000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Cryo-EM Structures of a Gonococcal Multidrug Efflux Pump Illuminate a Mechanism of Drug Recognition and Resistance"],"prefix":"10.1128","volume":"11","author":[{"given":"Meinan","family":"Lyu","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell A.","family":"Moseng","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Reimche","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA"},{"name":"Emory Antibiotic Resistance Center, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concerta L.","family":"Holley","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA"},{"name":"Emory Antibiotic Resistance Center, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijaya","family":"Dhulipala","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA"},{"name":"Emory Antibiotic Resistance Center, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Chia","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William M.","family":"Shafer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA"},{"name":"Emory Antibiotic Resistance Center, Emory University School of Medicine, Atlanta, Georgia, USA"},{"name":"Laboratories of Microbial Pathogenesis, VA Medical Center, Decatur, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5912-1227","authenticated-orcid":false,"given":"Edward W.","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.169"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00010-14"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143304"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2018.23.23.1800287"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2015.02.012"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90366-1"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm434"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1464"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.14.4162-4165.1995"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06424.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg031"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-7-2127"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02325.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-7-2117"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097903"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097475"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02281-18"},{"key":"e_1_3_3_19_2","first-page":"107","article-title":"The RND permease superfamily: an ancient, ubiquitous and diverse family that includes human disease and development proteins","volume":"1","author":"Tseng TT","year":"1999","unstructured":"Tseng TT, Gratwick KS, Kollman J, Park D, Nies DH, Goffeau A, Saier MH, Jr. 1999. The RND permease superfamily: an ancient, ubiquitous and diverse family that includes human disease and development proteins. 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