{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:37:32Z","timestamp":1785418652100,"version":"3.56.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-18-CE15-0007"],"award-info":[{"award-number":["ANR-18-CE15-0007"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-0062-IBEID"],"award-info":[{"award-number":["ANR-10-LABX-0062-IBEID"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2026,7,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Mosquito larvae rely on a living microbiota for normal development because the microbiota supplies essential nutrients, particularly vitamins. Beyond vitamin provision, transcriptomic data suggest that the microbiota also supports other key nutritional processes. Here, we explored these roles by conducting a metabolomics analysis on\n                      <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                      third instar larvae following microbiota depletion. We sampled larvae and dissected guts 12 and 20 h post-decolonization, and analysed methanol-soluble metabolites using untargeted gas chromatography\u2013mass spectrometry. Our findings reveal a pronounced impact of gut microbial presence on several metabolites involved in the tricarboxylic acid cycle and the uricolytic pathway. Germ-free larvae also had a lower quantity of cholesterol in their guts, and their long-chain fatty acid profile was altered in their guts and whole larvae. Sterols, including cholesterol, are essential precursors for the production of the molting hormone 20-hydroxyecdysone. We therefore tested how supplementing exogenous cholesterol affects the development of germ-free larvae. The effects proved to be highly concentration-dependent, ranging from a marginally significant increase in successful development to adulthood at low concentrations, to a pronounced developmental impairment at higher concentrations. Moreover, bacteria deficient in fatty acid beta-oxidation had a significantly lower ability to support larval development. Together, the observed alterations suggest that microbiota-deprived larvae exhibit a downregulation of metabolic processes related to energy production, nitrogen excretion, and sterol metabolism, likely due to the absence of microbiota-derived vitamins essential for these central metabolic functions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Mosquito larvae depend on gut microbiota for normal growth because microbes supply essential nutrients, particularly B vitamins. To explore microbial roles beyond vitamin provision, we analysed metabolic changes in\n                      <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                      larvae after microbiota removal using gas chromatography-mass spectrometry. Germ-free larvae exhibited decreased levels of metabolites associated with the tricarboxylic acid cycle and uricolytic pathway, indicating a general slowdown in metabolic activity and nitrogen waste processing. Additionally, the absence of a microbiota affected cholesterol and fatty acid metabolism. To validate these findings, we found that supplementing germ-free larvae with low levels of cholesterol modestly improved their development. In contrast, larvae colonized with bacteria deficient in fatty acid metabolism exhibited significantly reduced developmental success. Overall, the findings show that removing the microbiota downregulates key metabolic pathways related to energy production, nitrogen excretion, and sterol metabolism, highlighting that bacterial vitamins and fatty acid degradation are vital for mosquito larval development and successful transformation into adults.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.01035-26","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T13:00:21Z","timestamp":1781269221000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["The microbiota affects energy production, nitrogen excretion, and sterol metabolism in mosquito larvae"],"prefix":"10.1128","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7608-2650","authenticated-orcid":false,"given":"Ottavia","family":"Romoli","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0495fxg12","id-type":"ROR","asserted-by":"publisher"}],"name":"Microbiota of Insect Vectors Group, Institut Pasteur de la Guyane","place":["Cayenne, French Guiana"]},{"id":[{"id":"https:\/\/ror.org\/0495fxg12","id-type":"ROR","asserted-by":"publisher"}],"name":"Viruses and RNA interference Unit, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR3569","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanouk","family":"Epelboin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0495fxg12","id-type":"ROR","asserted-by":"publisher"}],"name":"Microbiota of Insect Vectors Group, Institut Pasteur de la Guyane","place":["Cayenne, French 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