{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:13:21Z","timestamp":1792635201759,"version":"4.5.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2014,5,27]],"date-time":"2014-05-27T00:00:00Z","timestamp":1401148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Acinetobacter baumannii<\/jats:named-content>\n                    is recognized as an emerging bacterial pathogen because of traits such as prolonged survival in a desiccated state, effective nosocomial transmission, and an inherent ability to acquire antibiotic resistance genes. A pressing need in the field of\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    research is a suitable model strain that is representative of current clinical isolates, is highly virulent in established animal models, and can be genetically manipulated. To identify a suitable strain, a genetically diverse set of recent U.S. military clinical isolates was assessed. Pulsed-field gel electrophoresis and multiplex PCR determined the genetic diversity of 33\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    isolates. Subsequently, five representative isolates were tested in murine pulmonary and\n                    <jats:named-content content-type=\"genus-species\">Galleria mellonella<\/jats:named-content>\n                    models of infection. Infections with one strain, AB5075, were considerably more severe in both animal models than those with other isolates, as there was a significant decrease in survival rates. AB5075 also caused osteomyelitis in a rat open fracture model, while another isolate did not. Additionally, a Tn\n                    <jats:italic>5<\/jats:italic>\n                    transposon library was successfully generated in AB5075, and the insertion of exogenous genes into the AB5075 chromosome via Tn\n                    <jats:italic>7<\/jats:italic>\n                    was completed, suggesting that this isolate may be genetically amenable for research purposes. Finally, proof-of-concept experiments with the antibiotic rifampin showed that this strain can be used in animal models to assess therapies under numerous parameters, including survival rates and lung bacterial burden. We propose that AB5075 can serve as a model strain for\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    pathogenesis due to its relatively recent isolation, multidrug resistance, reproducible virulence in animal models, and genetic tractability.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    The incidence of\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    infections has increased over the last decade, and unfortunately, so has antibiotic resistance in this bacterial species.\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    is now responsible for more than 10% of all hospital-acquired infections in the United States and has a &gt;50% mortality rate in patients with sepsis and pneumonia. Most research on the pathogenicity of\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    focused on isolates that are not truly representative of current multidrug-resistant strains isolated from patients. After screening of a panel of isolates in different\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    assays, the strain AB5075 was selected as more suitable for research because of its antibiotic resistance profile and increased virulence in animal models. Moreover, AB5075 is susceptible to tetracycline and hygromycin, which makes it amenable to genetic manipulation. Taken together, these traits make AB5075 a good candidate for use in studying virulence and pathogenicity of this species and testing novel antimicrobials.\n                  <\/jats:p>","DOI":"10.1128\/mbio.01076-14","type":"journal-article","created":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T00:26:42Z","timestamp":1401236802000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":340,"title":["AB5075, a Highly Virulent Isolate of Acinetobacter baumannii, as a Model Strain for the Evaluation of Pathogenesis and Antimicrobial Treatments"],"prefix":"10.1128","volume":"5","author":[{"given":"Anna C.","family":"Jacobs","sequence":"first","affiliation":[{"name":"Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell G.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad C.","family":"Black","sequence":"additional","affiliation":[{"name":"Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Kessler","sequence":"additional","affiliation":[{"name":"Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lily P.","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christin N.","family":"McQueary","sequence":"additional","affiliation":[{"name":"Department of Wound Infections, Walter 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