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In some\n            <jats:italic>Actinobacteria<\/jats:italic>\n            , defects in protein O-glycosylation have been shown to retard growth and result in hypersensitivity to cell wall-targeting antibiotics, suggesting that this modification is important for maintaining cell wall structure. Here, we have characterized the glycoproteome in\n            <jats:named-content content-type=\"genus-species\">Streptomyces coelicolor<\/jats:named-content>\n            and shown that glycoproteins have diverse roles, including those related to solute binding, ABC transporters, and cell wall biosynthesis. We have generated mutants encoding two putative cell wall-active glycoproteins and shown them to be hypersensitive to cell wall-targeting antibiotics. These findings strongly suggest that both glycoproteins are required for maintaining cell wall integrity and that glycosylation affects enzyme function.\n          <\/jats:p>","DOI":"10.1128\/mbio.01092-19","type":"journal-article","created":{"date-parts":[[2019,6,24]],"date-time":"2019-06-24T13:54:27Z","timestamp":1561384467000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of the Streptomyces coelicolor Glycoproteome Reveals Glycoproteins Important for Cell Wall Biogenesis"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2466-6918","authenticated-orcid":false,"given":"Tessa","family":"Keenan","sequence":"first","affiliation":[{"name":"Department of Biology, University of York, York, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Dowle","sequence":"additional","affiliation":[{"name":"Bioscience Technology Facility, University of York, York, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel","family":"Bates","sequence":"additional","affiliation":[{"name":"Bioscience Technology Facility, University of York, York, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4150-0496","authenticated-orcid":false,"given":"Margaret C. 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