{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:08:02Z","timestamp":1792642082085,"version":"4.5.0"},"reference-count":78,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T00:00:00Z","timestamp":1592870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T00:00:00Z","timestamp":1593475200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI101171"],"award-info":[{"award-number":["R01 AI101171"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["F32 AI122516"],"award-info":[{"award-number":["F32 AI122516"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35 GM118157"],"award-info":[{"award-number":["R35 GM118157"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32 GM131994"],"award-info":[{"award-number":["T32 GM131994"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["K99 HL143441"],"award-info":[{"award-number":["K99 HL143441"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2020,6,30]]},"abstract":"<jats:p>\n                    Although hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) has long been known as a respiratory poison, recent reports in numerous bacterial pathogens reveal that H\n                    <jats:sub>2<\/jats:sub>\n                    S and more downstream oxidized forms of sulfur collectedly termed reactive sulfur species (RSS) function as antioxidants to combat host efforts to clear the infection. Here, we present a comprehensive analysis of the transcriptional and proteomic response of\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    to exogenous sulfide as a model for how this important human pathogen manages sulfide\/RSS homeostasis. We show that\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    is unique in that it encodes two independent persulfide sensing and detoxification pathways that govern the speciation of bioactive sulfur in cells. The secondary persulfide sensor, BigR, impacts the expression of biofilm-associated genes; in addition, we identify two other transcriptional regulators known or projected to regulate biofilm formation, BfmR and Crp, as highly persulfidated in sulfide-exposed cells. These findings significantly strengthen the connection between sulfide homeostasis and biofilm formation in an important human pathogen.\n                  <\/jats:p>","DOI":"10.1128\/mbio.01254-20","type":"journal-article","created":{"date-parts":[[2020,6,22]],"date-time":"2020-06-22T10:09:27Z","timestamp":1592820567000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["The Response of\n                    <i>Acinetobacter baumannii<\/i>\n                    to Hydrogen Sulfide Reveals Two Independent Persulfide-Sensing Systems and a Connection to Biofilm Regulation"],"prefix":"10.1128","volume":"11","author":[{"given":"Brenna J. C.","family":"Walsh","sequence":"first","affiliation":[{"name":"Department of Chemistry, Indiana University, Bloomington, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiefei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Indiana University, Bloomington, Indiana, USA"},{"name":"Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine A.","family":"Edmonds","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Indiana University, Bloomington, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren D.","family":"Palmer","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, and Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University Medical Center, Nashville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory for Biological Mass Spectrometry, Indiana University, Bloomington, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan C.","family":"Trinidad","sequence":"additional","affiliation":[{"name":"Laboratory for Biological Mass Spectrometry, Indiana University, Bloomington, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric P.","family":"Skaar","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, and Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University Medical Center, Nashville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2342-1620","authenticated-orcid":false,"given":"David P.","family":"Giedroc","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Indiana University, Bloomington, Indiana, USA"},{"name":"Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00055"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00148"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0190599"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30753-3"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1209855"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00272-18"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1703576114"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10785-x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-14132-y"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1039\/c7sc00873b"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.7b00090"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321232111"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00298"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1834"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b07338"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00205"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.8b00463"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.008883"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-409547-2.14699-2"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.288423"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13726"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12835"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.8b00230"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1614133114"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.234039"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13725"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02385"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13372"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00082-17"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b00584"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.6b00714"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008198"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1039\/c8cc01339j"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1125603"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00413-18"},{"key":"e_1_3_3_37_2","doi-asserted-by":"crossref","unstructured":"Capdevila DA Walsh BJC Zhang Y Dietrich C Gonzalez-Gutierrez G Giedroc DP. 2020. Structural determinants of persulfide-sensing specificity in a dithiol-based transcriptional regulator. BioRxiv doi:10.1101\/2020.03.22.001966.","DOI":"10.1101\/2020.03.22.001966"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00352-10"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781683670285.ch12"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13511"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.652537"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2019.02.011"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2014.11.008"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2017.02.021"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101293"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00618-19"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi2004119"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01586.x"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.12.3730-3742.1999"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10067"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1500968"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2019.7777"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5776"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom7010033"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02679"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01650-14"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/019471-0"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2018.02.002"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001987"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-015-0440-6"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08560"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00164"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14029"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1614028114"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2014.06.017"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2016.06.009"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00767-15"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01929"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000623"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1252"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.149.1.173-180.1982"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1653449"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1263\/jbb.93.192"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00168-10"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(90)90494-c"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2010.07.002"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2646"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/009845-0"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.01254-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.01254-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T21:53:24Z","timestamp":1646690004000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.01254-20"}},"subtitle":[],"editor":[{"given":"Gisela","family":"Storz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,6,30]]},"references-count":78,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,6,30]]}},"alternative-id":["10.1128\/mBio.01254-20"],"URL":"https:\/\/doi.org\/10.1128\/mbio.01254-20","relation":{},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,30]]},"assertion":[{"value":"2020-05-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01254-20"}}