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It adapts to the different host niches through a complex gene regulatory network. We show here that the Rho transcription termination factor, which represses pervasive antisense transcription in various bacteria, including\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            , plays a role in controlling SaeRS-dependent virulence gene expression. A Rho-deficient strain produces larger amounts of secreted virulence factors\n            <jats:italic>in vitro<\/jats:italic>\n            and shows increased virulence in mice. We also show that treatment of\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            with the antibiotic bicyclomycin, which inhibits Rho activity and is effective against Gram-negative bacteria, induces the same changes in the proteome as observed in the Rho-deficient strain. Our results reveal for the first time a link between transcription termination and virulence regulation in\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            , which implies a novel mechanism by which an antibiotic can modulate the expression of virulence factors.\n          <\/jats:p>","DOI":"10.1128\/mbio.01332-18","type":"journal-article","created":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T05:27:37Z","timestamp":1537248457000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Inhibition of Rho Activity Increases Expression of SaeRS-Dependent Virulence Factor Genes in Staphylococcus aureus, Showing a Link between Transcription Termination, Antibiotic Action, and Virulence"],"prefix":"10.1128","volume":"9","author":[{"given":"Anna","family":"Nagel","sequence":"first","affiliation":[{"name":"Interfaculty Institute for Genetics and 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