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This report describes the successful adaptation of an important tool that has been lacking in\n            <jats:italic>Chlamydia<\/jats:italic>\n            studies: transposon mutagenesis. This advance enabled the generation of 105 insertional mutants, demonstrating that numerous gene products are not essential for\n            <jats:italic>in vitro<\/jats:italic>\n            growth. Mammalian infections using these mutants revealed that several gene products are important for infections\n            <jats:italic>in vivo<\/jats:italic>\n            . Moreover, this tool enabled the investigation and discovery of a gene critical for lateral gene transfer; a process fundamental to the evolution of bacteria and likely for\n            <jats:italic>Chlamydia<\/jats:italic>\n            as well. The development of transposon mutagenesis for\n            <jats:italic>Chlamydia<\/jats:italic>\n            has broad impact for the field and for the discovery of genes associated with selected phenotypes, providing an additional avenue for the discovery of molecular mechanisms used for pathogenesis and for a more thorough understanding of this important pathogen.\n          <\/jats:p>","DOI":"10.1128\/mbio.01343-19","type":"journal-article","created":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T12:29:01Z","timestamp":1565008141000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer"],"prefix":"10.1128","volume":"10","author":[{"given":"Scott D.","family":"LaBrie","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zo\u00eb E.","family":"Dimond","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly S.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srishti","family":"Baid","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Wickstrum","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Suchland","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington\u2014Seattle, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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