{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:22:39Z","timestamp":1785406959203,"version":"3.56.0"},"reference-count":95,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,8,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Streptococcus dysgalactiae<\/jats:italic>\n                      subspecies\n                      <jats:italic toggle=\"yes\">equisimilis<\/jats:italic>\n                      (SDSE) is a gram-positive bacterial pathogen capable of causing various infections in humans. Recently, isolates of SDSE\n                      <jats:italic toggle=\"yes\">emm<\/jats:italic>\n                      type\n                      <jats:italic toggle=\"yes\">stG62647<\/jats:italic>\n                      have emerged as a cause of severe invasive infections, including necrotizing myositis. However, the molecular processes underlying these infections remain poorly understood. To address this gap, we performed RNAseq analysis to examine SDSE gene transcript levels during experimental necrotizing myositis infection in non-human primates, animals phylogenetically closely related to humans. We analyzed the transcriptomes of two related SDSE\n                      <jats:italic toggle=\"yes\">stG62647<\/jats:italic>\n                      human isolates (MGCS36044 and MGCS36089) during necrotizing myositis infection in six non-human primates. The transcriptome from\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      growth in nutrient-rich media differed considerably from that of SDSE bacteria grown\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      in experimental necrotizing myositis, with 254 genes differentially expressed, indicating extensive genetic adaptation in infected skeletal muscle. Notably, we observed a marked upregulation of\n                      <jats:italic toggle=\"yes\">ihk-irr<\/jats:italic>\n                      genes encoding a two-component regulatory system that promotes evasion of phagocytosis and resistance to killing by human polymorphonuclear leukocytes in\n                      <jats:italic toggle=\"yes\">Streptococcus pyogenes<\/jats:italic>\n                      . Similarly, genes comprising the\n                      <jats:italic toggle=\"yes\">sag<\/jats:italic>\n                      operon encoding the streptolysin S cytolytic toxin virulence factor demonstrated very high transcript abundance\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      . Additionally, we present evidence that a 40-nt deletion in\n                      <jats:italic toggle=\"yes\">fasB<\/jats:italic>\n                      alters expression of\n                      <jats:italic toggle=\"yes\">ska<\/jats:italic>\n                      , encoding streptokinase. Collectively, our data provide new insights into the SDSE genes transcribed\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      , thereby enhancing our understanding of the molecular basis of pathogen and primate host interactions. The SDSE genes identified in this study offer promising targets for future studies on molecular pathogenesis and therapeutic interventions.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Streptococcus dysgalactiae<\/jats:italic>\n                        subspecies\n                        <jats:italic toggle=\"yes\">equisimilis<\/jats:italic>\n                        (SDSE) has emerged as an increasingly important bacterial pathogen causing serious invasive infections in humans worldwide. Despite its clinical importance, the mechanisms through which SDSE causes infections remain poorly understood, and no licensed vaccine currently exists. SDSE can cause necrotizing myositis, an infection with high morbidity and mortality. We used a primate infection model and bacterial transcriptome analysis to gain new understanding of the molecular events contributing to SDSE pathogenesis in necrotizing myositis. Our results provide extensive new information about the transcriptome of SDSE\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        and reveal numerous potential targets for future therapeutic and vaccine research.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.01349-25","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T13:00:07Z","timestamp":1753189207000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Analysis of\n                    <i>Streptococcus dysgalactiae<\/i>\n                    subspecies\n                    <i>equisimilis<\/i>\n                    gene transcripts during experimental primate necrotizing myositis"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1383-8702","authenticated-orcid":true,"given":"Jesus M.","family":"Eraso","sequence":"first","affiliation":[{"name":"Laboratory for Molecular and Translational Human Infectious Diseases Research, Center for Infectious Diseases, Houston Methodist Research Institute","place":["Houston, USA"]},{"name":"Department of Pathology and Genomic Medicine, Houston Methodist Hospital","place":["Houston, USA"]},{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5295-4178","authenticated-orcid":true,"given":"Randall J.","family":"Olsen","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular and Translational Human Infectious Diseases Research, Center for Infectious Diseases, Houston Methodist Research Institute","place":["Houston, USA"]},{"name":"Department of Pathology and Genomic Medicine, Houston Methodist Hospital","place":["Houston, USA"]},{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3043-5307","authenticated-orcid":true,"given":"S. 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