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Although an acute CHIKV infection is rarely fatal, many patients suffer from debilitating chronic arthralgia for years. Antivirals against chikungunya and other alphaviruses have been identified\n            <jats:italic>in vitro<\/jats:italic>\n            , but to date none have been shown to be efficacious and have been licensed for human use. Here, we investigated a small molecule, berberine chloride (BBC), and showed that it inhibited infectious virus production by several alphaviruses including CHIKV. BBC acted on a late step in the alphavirus exit pathway, namely the formation of the nucleocapsid containing the infectious viral RNA. Better understanding of nucleocapsid formation and its inhibition by BBC will provide important information on the mechanisms of infectious alphavirus production and may enable their future targeting in antiviral strategies.\n          <\/jats:p>","DOI":"10.1128\/mbio.01382-20","type":"journal-article","created":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T14:23:20Z","timestamp":1593440600000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Berberine Chloride is an Alphavirus Inhibitor That Targets Nucleocapsid Assembly"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0637-6968","authenticated-orcid":false,"given":"Judy J.","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3605-5030","authenticated-orcid":false,"given":"Rebecca S.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7395-4791","authenticated-orcid":false,"given":"Margaret","family":"Kielian","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"629","volume-title":"Fields virology","author":"Kuhn RJ","year":"2013","unstructured":"Kuhn RJ. 2013. 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