{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T16:49:01Z","timestamp":1788367741244,"version":"build-2803163510"},"reference-count":8,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["AI139071"],"award-info":[{"award-number":["AI139071"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["AI132356"],"award-info":[{"award-number":["AI132356"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2023,10,31]]},"abstract":"ABSTRACT<\/jats:title>\n \n Folic acid and its derivatives are required for the synthesis of purines, pyrimidines, and some amino acids. Antifolate antibiotics that target the folic acid metabolism pathway are commonly used for the treatment of listeriosis caused by the intracellular pathogen\n Listeria monocytogenes<\/jats:italic>\n (\n Lm<\/jats:italic>\n ). In recent work in\n mBio<\/jats:italic>\n , Feng et al. sought to understand the role of folic acid metabolism in\n Lm<\/jats:italic>\n virulence (Y. Feng, S. Chang, D. A. Portnoy, 2023, mBio\n https:\/\/doi.org\/10.1128\/mbio.01074-23<\/jats:ext-link>\n ). The authors discovered that N-formylmethionine, an amino acid utilized by bacteria to initiate protein synthesis, is crucial for\n Lm<\/jats:italic>\n intracellular growth and pathogenesis. Surprisingly, purines and thymidine were found to be dispensable for\n Lm<\/jats:italic>\n infection. Together these results demonstrate that while\n Lm<\/jats:italic>\n can obtain many essential nutrients from the host cytosol, including purines and most amino acids, it requires N-formylmethionine biosynthesis to properly regulate translation initiation during infection.\n <\/jats:p>","DOI":"10.1128\/mbio.01385-23","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T14:04:06Z","timestamp":1694700246000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Listeria monocytogenes<\/i>\n folate metabolism is required to generate N-formylmethionine during infection"],"prefix":"10.1128","volume":"14","author":[{"given":"Qing","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Medicine, University of Washington , Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0770-7784","authenticated-orcid":false,"given":"Michelle L.","family":"Reniere","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, University of Washington , Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01074-23"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00207-22"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00156"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2022.03.028"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV120.011985"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00162-13"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep00786"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a028324"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.01385-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.01385-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T18:27:05Z","timestamp":1723400825000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mbio.01385-23"}},"subtitle":[],"editor":[{"given":"Nancy E.","family":"Freitag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,10,31]]},"references-count":8,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,10,31]]}},"alternative-id":["10.1128\/mbio.01385-23"],"URL":"https:\/\/doi.org\/10.1128\/mbio.01385-23","relation":{},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,31]]},"assertion":[{"value":"2023-09-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01385-23"}}