{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T06:31:18Z","timestamp":1768631478273,"version":"3.49.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T00:00:00Z","timestamp":1532995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,9,5]],"date-time":"2018-09-05T00:00:00Z","timestamp":1536105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2018,9,5]]},"abstract":"\n Syphilis research has been severely limited by the necessity to propagate\n Treponema pallidum<\/jats:named-content>\n in vivo<\/jats:italic>\n in rabbits. After decades of erroneous or irreproducible reports of cultivation of\n T.\u00a0pallidum<\/jats:named-content>\n , the recent very convincing report of its successful long-term\n in vitro<\/jats:italic>\n propagation opens numerous opportunities for development of genetic tools for studying pathogenesis and protein function, antigenic variation, and surface exposure of antigens.\n <\/jats:p>","DOI":"10.1128\/mbio.01417-18","type":"journal-article","created":{"date-parts":[[2018,7,30]],"date-time":"2018-07-30T09:59:22Z","timestamp":1532944762000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["New Tools for Syphilis Research"],"prefix":"10.1128","volume":"9","author":[{"given":"Sheila A.","family":"Lukehart","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"},{"name":"Department of Global Health, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Sexually transmitted disease surveillance 2016","author":"Centers for Disease Control and Prevention","year":"2017","unstructured":"Centers for Disease Control and Prevention. 2017. 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