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Although HSK immunopathology of the cornea is well documented in animal models, events preceding this abnormal inflammatory cascade are poorly understood. In this study, we have examined the activation of pathological CD4<jats:sup>+<\/jats:sup>T cells in the development of HSK. Dendritic cell autophagy (DC-autophagy) is an important pathway regulating major histocompatibility complex class II (MHCII)-dependent antigen presentation and proper CD4<jats:sup>+<\/jats:sup>T cell activation during infectious diseases. Using DC-autophagy-deficient mice, we found that DC-autophagy significantly and specifically contributes to HSK disease without impacting early innate immune infiltration, viral clearance, or host survival. Instead, the observed phenotype was attributable to the abrogated activation of CD4<jats:sup>+<\/jats:sup>T cells and reduced inflammation in HSK lesions. We conclude that DC-autophagy is an important contributor to primary HSK immunopathology upstream of CD4<jats:sup>+<\/jats:sup>T cell activation.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>Herpetic stromal keratitis (HSK) is the leading cause of infectious blindness in the United States and a rising cause worldwide. HSK is induced by herpes simplex virus 1 but is considered a disease of inappropriately sustained inflammation driven by CD4<jats:sup>+<\/jats:sup>T cells. In this study, we investigated whether pathways preceding CD4<jats:sup>+<\/jats:sup>T cell activation affect disease outcome. We found that autophagy in dendritic cells significantly contributed to the incidence of HSK. Dendritic cell autophagy did not alter immune control of the virus or neurological disease but specifically augmented CD4<jats:sup>+<\/jats:sup>T cell activation and pathological corneal inflammation. This study broadens our understanding of the immunopathology that drives HSK and implicates the autophagy pathway as a new target for therapeutic intervention against this incurable form of infectious blindness.<\/jats:p>","DOI":"10.1128\/mbio.01426-15","type":"journal-article","created":{"date-parts":[[2015,10,28]],"date-time":"2015-10-28T07:19:16Z","timestamp":1446016756000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Dendritic Cell Autophagy Contributes to Herpes Simplex Virus-Driven Stromal Keratitis and Immunopathology"],"prefix":"10.1128","volume":"6","author":[{"given":"Yike","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotang","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Saint Louis University, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick M.","family":"Stuart","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Saint Louis University, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Leib","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200101000-00001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2012.01.005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(81)90285-3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/09286586.2014.966848"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1989.01070020221029"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2010.187"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.26.3.1164-1171.1979"},{"key":"e_1_3_2_9_2","first-page":"938","article-title":"Role of T-lymphocytes in the pathogenesis of herpetic stromal keratitis","volume":"25","author":"Russell RG","year":"1984","unstructured":"Russell RG, Nasisse MP, Larsen HS, Rouse BT. 1984. 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