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The lack of viable\n            <jats:italic>in vitro\/ex vivo<\/jats:italic>\n            PCP models has greatly hindered the progress in studying the biology of these fungi, the host\/pathogen interactions, as well as antifungal susceptibility testing. In this study, we show the utility of precision-cut lung slices (PCLS) to support the survival of\n            <jats:italic>Pneumocystis murina in vitro<\/jats:italic>\n            . We cultured PCLS tissue derived from wild type and immunocompromised mice with a\n            <jats:italic>P. murina<\/jats:italic>\n            inoculum in submerged or air-liquid interface models for up to 14 days. We isolated total RNA from the cultured lung tissues at days 0, 3, 7, 10, and 14 and analyzed for the expression of host lung genes and\n            <jats:italic>P. murina<\/jats:italic>\n            genes (\n            <jats:italic>Gsc1<\/jats:italic>\n            for asci and\n            <jats:italic>Sp<\/jats:italic>\n            for trophs) by real-time quantitative PCR. When cultured in media alone,\n            <jats:italic>P. murina<\/jats:italic>\n            died gradually within a few days. However, when cultured on PCLS, both the troph and ascus forms survived throughout the incubation period of 2 weeks. Moreover, immunohistochemistry staining of\n            <jats:italic>P. murina<\/jats:italic>\n            inoculated PCLS sections using polyclonal anti-\n            <jats:italic>Pneumocystis<\/jats:italic>\n            sera and showed evidence of fungal aggregation and possible biofilm formation. Additionally,\n            <jats:italic>in vitro<\/jats:italic>\n            (PCLS) antibiotic susceptibility testing using commonly used antifungal drugs confirmed successful targeting of the troph and ascus forms by trimethoprim sulfamethoxazole and the ascus form by echinocandins.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Our study reveals the potential of precision-cut lung slices as an\n              <jats:italic>ex vivo<\/jats:italic>\n              platform to study the growth\/survival of\n              <jats:italic>Pneumocystis<\/jats:italic>\n              spp. that can facilitate the development of new anti-fungal drugs.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.01464-23","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T13:50:36Z","timestamp":1703080236000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Precision-cut lung slices as an\n            <i>ex vivo<\/i>\n            model to study\n            <i>Pneumocystis murina<\/i>\n            survival and antimicrobial susceptibility"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6063-7606","authenticated-orcid":false,"given":"Ferris T.","family":"Munyonho","sequence":"first","affiliation":[{"name":"Center for Translational Research in Infection and Inflammation Tulane School of Medicine, New Orleans, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D. 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