{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:27:58Z","timestamp":1790530078570,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,12,19]],"date-time":"2017-12-19T00:00:00Z","timestamp":1513641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T00:00:00Z","timestamp":1514505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM103612"],"award-info":[{"award-number":["R01GM103612"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"HHS | NIH | National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["DC003915"],"award-info":[{"award-number":["DC003915"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2017,12,29]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Biofilms formed in the middle ear by nontypeable\n            <jats:italic>Haemophilus influenzae<\/jats:italic>\n            (NTHI) are central to the chronicity, recurrence, and refractive nature of otitis media (OM). However, mechanisms that underlie the emergence of specific NTHI biofilm structures are unclear. We combined computational analysis tools and\n            <jats:italic>in silico<\/jats:italic>\n            modeling rooted in statistical physics with confocal imaging of NTHI biofilms formed\n            <jats:italic>in vitro<\/jats:italic>\n            during static culture in order to identify mechanisms that give rise to distinguishing morphological features. Our analysis of confocal images of biofilms formed by NTHI strain 86-028NP using pair correlations of local bacterial densities within sequential planes parallel to the substrate showed the presence of fractal structures of short length scales (\u226410\u00a0\u03bcm). The\n            <jats:italic>in silico<\/jats:italic>\n            modeling revealed that extracellular DNA (eDNA) and type IV pilus (Tfp) expression played important roles in giving rise to the fractal structures and allowed us to predict a substantial reduction of these structures for an isogenic mutant (\u0394\n            <jats:italic>comE<\/jats:italic>\n            ) that was significantly compromised in its ability to release eDNA into the biofilm matrix and had impaired Tfp function. This prediction was confirmed by analysis of confocal images of\n            <jats:italic>in vitro<\/jats:italic>\n            \u0394\n            <jats:italic>comE<\/jats:italic>\n            strain biofilms. The fractal structures potentially generate niches for NTHI survival in the hostile middle ear microenvironment by dramatically increasing the contact area of the biofilm with the surrounding environment, facilitating nutrient exchange, and by generating spatial positive feedback to quorum signaling.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            NTHI is a major bacterial pathogen for OM, which is a common ear infection in children worldwide. Chronic OM is associated with bacterial biofilm formation in the middle ear; therefore, knowledge of the mechanisms that underlie NTHI biofilm formation is important for the development of therapeutic strategies for NTHI-associated OM. Our combined approach using confocal imaging of NTHI biofilms formed\n            <jats:italic>in vitro<\/jats:italic>\n            and mathematical tools for analysis of pairwise density correlations and agent-based modeling revealed that eDNA and Tfp expression were important factors in the development of fractal structures in NTHI biofilms. These structures may help NTHI survive in hostile environments, such as the middle ear. Our\n            <jats:italic>in silico<\/jats:italic>\n            model can be used in combination with laboratory or animal modeling studies to further define the mechanisms that underlie NTHI biofilm development during OM and thereby guide the rational design of, and optimize time and cost for, benchwork and preclinical studies.\n          <\/jats:p>","DOI":"10.1128\/mbio.01466-17","type":"journal-article","created":{"date-parts":[[2018,1,5]],"date-time":"2018-01-05T13:51:59Z","timestamp":1515160319000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Extracellular DNA and Type IV Pilus Expression Regulate the Structure and Kinetics of Biofilm Formation by Nontypeable\n            <i>Haemophilus influenzae<\/i>"],"prefix":"10.1128","volume":"8","author":[{"given":"Jayajit","family":"Das","sequence":"first","affiliation":[{"name":"Battelle Center for Mathematical Medicine, The Research Institute at the 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Rosas","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, The Research Institute at the Nationwide Children\u2019s Hospital, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A.","family":"Jurcisek","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, The Research Institute at the Nationwide Children\u2019s Hospital, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Ray","sequence":"additional","affiliation":[{"name":"Battelle Center for Mathematical Medicine, The Research Institute at the Nationwide Children's Hospital, Columbus, Ohio, USA"},{"name":"Department of Pediatrics, the Ohio State University College of Medicine, Columbus, Ohio, USA"},{"name":"Department of Biophysics Graduate Program, the Ohio State University, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren O.","family":"Bakaletz","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, The Research Institute at the Nationwide Children\u2019s Hospital, Columbus, Ohio, USA"},{"name":"Department of Pediatrics, the Ohio State University College of Medicine, Columbus, Ohio, 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